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Silver based raw materials for cosmetic purposes
Čaková, Adriana ; Ďubašáková, Mária (referee) ; Zemanová, Jana (advisor)
The purpose of this bachelor’s thesis was a research about silver based cosmetic products and determination of antimicrobial activity on certain species of microorganisms by chosen silver based cosmetic products. Theoretical part is focused on the problematics of silver based cosmetic products, on their main purpose, physically chemical characteristics and toxicological profile. In the theoretical part is also mentioned problematics of legislative, mainly the safety and real effectivity of tested cosmetic products. Experimental part was focused on determination of antimicrobial activity of chosen silver based cosmetic products. Two silver based cosmetic product were used to determined antimicrobial activity on selected microorganisms Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Kocuria rosea, Serratia marcescens a Candida vini. From result it is apparent, that both of used Cosmetic product showed antimicrobial activity, but not on every used microorganism.
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Development and preparation of antimcrobial nanostructure biomaterials
Drabíková, Nela ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
The presented diploma thesis deals with the optimalisation of preparation and the preparation of combined nanostructured antimicrobial biomaterials itself. In the theoretical part, a review focused on used materials and consequently preparation of nanoparticles and nanofibers was elaborated. Furthermore, the used antimicrobial substances – curcumin and ampicillin, and the principle of cytotoxicity assay were described.In practical part the optimalisation process is described. Furthermore the safety of prepared materials and used antimicrobial substances on HaCaT cell line was tested, in order to confirm their possible further use in cosmetic and pharmaceutical industry. Great part of the thesis deals with evaluation of the antimicrobial activity of used substances and prepared combined nanomaterials on multiple microorganisms from grampositive bacteria, gramnegative bacteria and yeasts. Also the release speed of antimicrobial substances from prepared nanomaterials was determined by spectrophotometer. The amount of released ampicillin from prepared nanomaterials was determined by liquid chromatography.
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Use of cell cultures to testing of natural substances of plant origin
Ručková, Michaela ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
The presented bachelor thesis is focused on preparing aqueous and oil extracts of plant origin, their characterization and testing of their cytotoxicity and genotoxicity. The theoretical part contains basic information about plants and kinds of spices or tea that were used. Description of used methods of liposome preparation and encapsulation of active substances, measurement of antioxidant activity, particle characterization, cultivation of cell cultures and the principle of cytotoxicity and genotoxicity tests used in this thesis is included. Phenolics and flavonoids content was determined using the spectrophotometry. Antioxidant activity was evaluated spectrophotometrically as well. By ultrasonic homogenization method, liposomes were prepared and active agents were encapsulated into these vesicles. Encapsulation efficiency and stability of active substances were measured by spectrophotometry. By DLS method, size of particles was determined. Particle stability was derived from the zeta potential value. Important part of the bachelor thesis was cultivation of human keratinocytes. The cytotoxicity of extracts and liposomes was assessed by MTT assay on human cell cultures. The genotoxicity of extracts and liposomes was defined using SOS Chromotest on model organism, genetically modified E. coli. In conclusion, the potential use of prepared liposomes in the cosmetic or pharmaceutical industry has been proposed.
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Preparation of skin masks with the anti-acne effect
Geierová, Kateřina ; Bendová, Agáta (referee) ; Márová, Ivana (advisor)
This bachelor thesis was focused on the preparation and characterization of nanofibers containing active agents with the aim of using these materials in anti-acne facial masks. The nanofibers were prepared from polyhydroxybutyrate solution and gelatin solution by electrospinning method. Among the active ingredients, eugenol and piperine were used to functionalize the nanofibers. In the theoretical part, the types of face masks, the individual active ingredients, the materials for the preparation of nanofibers, their preparation methods and their characterization were characterized. In the practical part, the antioxidant and antimicrobial activity of the active ingredients themselves was investigated. Subsequently, the release rate of selected active ingredients from the prepared nanofibers was monitored in three model environments. The antimicrobial activity of the nanofibers was monitored on three microorganisms, namely Staphylococcus epidermidis, Cutibacterium acnes and Escherichia coli.
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Development and preparation of antimcrobial nanostructure biomaterials
Drabíková, Nela ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
The presented diploma thesis deals with the optimalisation of preparation and the preparation of combined nanostructured antimicrobial biomaterials itself. In the theoretical part, a review focused on used materials and consequently preparation of nanoparticles and nanofibers was elaborated. Furthermore, the used antimicrobial substances – curcumin and ampicillin, and the principle of cytotoxicity assay were described.In practical part the optimalisation process is described. Furthermore the safety of prepared materials and used antimicrobial substances on HaCaT cell line was tested, in order to confirm their possible further use in cosmetic and pharmaceutical industry. Great part of the thesis deals with evaluation of the antimicrobial activity of used substances and prepared combined nanomaterials on multiple microorganisms from grampositive bacteria, gramnegative bacteria and yeasts. Also the release speed of antimicrobial substances from prepared nanomaterials was determined by spectrophotometer. The amount of released ampicillin from prepared nanomaterials was determined by liquid chromatography.
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Silver based raw materials for cosmetic purposes
Čaková, Adriana ; Ďubašáková, Mária (referee) ; Zemanová, Jana (advisor)
The purpose of this bachelor’s thesis was a research about silver based cosmetic products and determination of antimicrobial activity on certain species of microorganisms by chosen silver based cosmetic products. Theoretical part is focused on the problematics of silver based cosmetic products, on their main purpose, physically chemical characteristics and toxicological profile. In the theoretical part is also mentioned problematics of legislative, mainly the safety and real effectivity of tested cosmetic products. Experimental part was focused on determination of antimicrobial activity of chosen silver based cosmetic products. Two silver based cosmetic product were used to determined antimicrobial activity on selected microorganisms Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Kocuria rosea, Serratia marcescens a Candida vini. From result it is apparent, that both of used Cosmetic product showed antimicrobial activity, but not on every used microorganism.
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Use of cell cultures to testing of natural substances of plant origin
Ručková, Michaela ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
The presented bachelor thesis is focused on preparing aqueous and oil extracts of plant origin, their characterization and testing of their cytotoxicity and genotoxicity. The theoretical part contains basic information about plants and kinds of spices or tea that were used. Description of used methods of liposome preparation and encapsulation of active substances, measurement of antioxidant activity, particle characterization, cultivation of cell cultures and the principle of cytotoxicity and genotoxicity tests used in this thesis is included. Phenolics and flavonoids content was determined using the spectrophotometry. Antioxidant activity was evaluated spectrophotometrically as well. By ultrasonic homogenization method, liposomes were prepared and active agents were encapsulated into these vesicles. Encapsulation efficiency and stability of active substances were measured by spectrophotometry. By DLS method, size of particles was determined. Particle stability was derived from the zeta potential value. Important part of the bachelor thesis was cultivation of human keratinocytes. The cytotoxicity of extracts and liposomes was assessed by MTT assay on human cell cultures. The genotoxicity of extracts and liposomes was defined using SOS Chromotest on model organism, genetically modified E. coli. In conclusion, the potential use of prepared liposomes in the cosmetic or pharmaceutical industry has been proposed.
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