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Determination of volatile substances of natural extracts intended for cosmetic application
Paskevych, Olha ; Řezáčová, Veronika (referee) ; Mravcová, Ludmila (advisor)
Tato bakalářská práce je soustředěná na zkoumaní přírodních komerčně dostupných extraktů, které se používají do přírodní kosmetiky. Literární rešerše byla zaměřena na téma obsahu těkavých látek v přírodních extraktech používaných v kosmetice, jejich charakterizace a možnosti stanovení, problematika výskytu alergenů v přírodní kosmetice. Na základě tohoto přehledu byla navržena optimální metoda pro analýzu vzorků přírodních extraktů s přihlédnutím k dostupné analytické instrumentaci. Následující výtažky byly studovány se zaměřením na kvalitativní stanovení: mandle, aloe vera, hřebíček, Etiopská káva, zázvor, cibule.
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Isolation, characterization and application of active components of prickly pear and aloe vera
Kocúrová, Petra ; Vysoká, Marie (referee) ; Skoumalová, Petra (advisor)
The main goal of this bachelor thesis was the study of antioxidant and antibacterial effect of natural plant extracts, specifically from pulp and peel of leaf of Aloe vera and Opuntia ficus–indica. Liposomes with encapsulated active compounds were prepared and their use in food and cosmetics industry was analyzed. The theoretical part was focused on botanical with and phytochemical description of natural plant and their use in nutrition and medical practice. Also encapsulation and methods used to analyze encapsulated substances were described. In the experimental part basic characteristics of extracts from two kinds of plantusing ethanol as a solvent in its different concentration (0 %, 20 %, 40 %, 80 %) were analyzed. Mainly totalcontent of phenolics, flavonoids and total antioxidant activity were determined. Selected extracts were also encapsulated into liposomes and the encapsulation efficienty, size, stability and long–term stability of liposomes were determined. Antibacterial activity was tested against Grampositive Micrococcus luteus, Staphylococcus epidermidis and Propionibacterium acnes and Gramnegative Serratia marcescems a Escherichia coli. Moreover, antibacterial acvivity against yeast Candida glabrata was tested. Finally, 2 ethanol extracts of plant samples were tested in terms of cytotoxicity by using cell line Caco–2 and profile of selected phenolic compounds ere determined in the selected extract samples by using high performance liqiud chromatography. 20% ethanol solution was chosen as the most efficient extract solvent which contained the highest concentration of total phenolic content and concurrently all tested extracts showed antioxidant activity. However, there was minimal or none antibacterial effect. Prepared liposomes from extracts were stable. Also product for cosmetics and food industry was designed, specifically healing gel with moisturizing effect and paste made of liposomes as food supplement for athletes.
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Determination of volatile substances of natural extracts intended for cosmetic application
Paskevych, Olha ; Řezáčová, Veronika (referee) ; Mravcová, Ludmila (advisor)
Tato bakalářská práce je soustředěná na zkoumaní přírodních komerčně dostupných extraktů, které se používají do přírodní kosmetiky. Literární rešerše byla zaměřena na téma obsahu těkavých látek v přírodních extraktech používaných v kosmetice, jejich charakterizace a možnosti stanovení, problematika výskytu alergenů v přírodní kosmetice. Na základě tohoto přehledu byla navržena optimální metoda pro analýzu vzorků přírodních extraktů s přihlédnutím k dostupné analytické instrumentaci. Následující výtažky byly studovány se zaměřením na kvalitativní stanovení: mandle, aloe vera, hřebíček, Etiopská káva, zázvor, cibule.
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Isolation, characterization and application of active components of prickly pear and aloe vera
Kocúrová, Petra ; Vysoká, Marie (referee) ; Skoumalová, Petra (advisor)
The main goal of this bachelor thesis was the study of antioxidant and antibacterial effect of natural plant extracts, specifically from pulp and peel of leaf of Aloe vera and Opuntia ficus–indica. Liposomes with encapsulated active compounds were prepared and their use in food and cosmetics industry was analyzed. The theoretical part was focused on botanical with and phytochemical description of natural plant and their use in nutrition and medical practice. Also encapsulation and methods used to analyze encapsulated substances were described. In the experimental part basic characteristics of extracts from two kinds of plantusing ethanol as a solvent in its different concentration (0 %, 20 %, 40 %, 80 %) were analyzed. Mainly totalcontent of phenolics, flavonoids and total antioxidant activity were determined. Selected extracts were also encapsulated into liposomes and the encapsulation efficienty, size, stability and long–term stability of liposomes were determined. Antibacterial activity was tested against Grampositive Micrococcus luteus, Staphylococcus epidermidis and Propionibacterium acnes and Gramnegative Serratia marcescems a Escherichia coli. Moreover, antibacterial acvivity against yeast Candida glabrata was tested. Finally, 2 ethanol extracts of plant samples were tested in terms of cytotoxicity by using cell line Caco–2 and profile of selected phenolic compounds ere determined in the selected extract samples by using high performance liqiud chromatography. 20% ethanol solution was chosen as the most efficient extract solvent which contained the highest concentration of total phenolic content and concurrently all tested extracts showed antioxidant activity. However, there was minimal or none antibacterial effect. Prepared liposomes from extracts were stable. Also product for cosmetics and food industry was designed, specifically healing gel with moisturizing effect and paste made of liposomes as food supplement for athletes.
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