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Diagnostics of species complex in family Adelgidae by molecular markers.
CHUNDELOVÁ, Daniela
Five molecular markers (mitochondrial AT-control region and COI, nuclear ITS and markers from primary enosymbiont Buchnera sp. argS and rrs) were tested for use in species identification of 2 selected genuses of the Adelgidae family. Markers COI, argS and rrs were succesfuly amplified and sequenced. Aligned sequences of those three markers were used for reconstruction of dendrogram by Neighbour-Joining metod with Kimura 2-Parameter model. These markers are suitable for resolution of taxonomic relationship of genuses within family Adelgidae, although further testing of these markers in conjunction with other markers (like ITS or AT-control region) will be required to delineate sister species with higher precision.
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Optimization of cytogenetic techniques in a model aphid, \kur{Diuraphis noxia} (Aphididae)
VESELÁ, Jana
Several cytogenetical methods for investigation of karyotyp were used in an aphid species, Diuraphis noxia (Hemiptera: Aphididae). These methods (FISH with telomeric probe, FISH with rDNA probe, FISH with histone probe and silver staining) were optimized for application to a sister group Adelgidae, where they could be useful for better differentiation of species in particular stages of their complicated life cycle.
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Diagnostics of species complex in the family Adelgidae by molecular markers
JAROŠOVÁ, Andrea
Three molecular markers were tested for use in species identification of two selected genuses of the Adelgidae. Two mitochondrial markers were successfully amplified and sequenced, part of the COI gene and fragment between the COII and COIII genes. Their ability to distinguish species complexes was checked by statistical methods and by construction of phylogenetic trees.
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Identification of species within \kur{Adelgidae} by molecular markers
VĚCHTOVÁ, Pavlína
Four molecular markers (nuclear ITS, EF1 alpha and mitochondrial AT-Rich and COI) were tested for use in species identification of Adelgidae family. Markers EF1 alpha and COI were successfuly amplified and sequenced. Aligned sequences of those two markers were used for reconstruction of dendrogram by Minimum Evolution method with Kimura 2-Parameter model. Comparison of dendrogram with assumptions based on morfology and biology of Adelgids confirmed suitability of chosen molecular markers for reliable resolution of taxonomic relationship within Adelgidae, although further testing and markers will be required to delineate sister species with higher precision.
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