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AHL genes in plant development
Škrabálková, Eliška ; Širl, Marek (advisor) ; Ortmannová, Jitka (referee)
Plant development is a complex process where different factors come into play. The expression of certain genes, which determine basic plant structure and its signaling pathways, is also of great importance. The AHL gene family, which in case of Arabidopsis thaliana includes 29 members, is one of those determinators that have an impact on plant development on several levels. As far as structure is concerned, the AHL proteins are typically composed of two parts - the DNA-bonding AT-hook motif and PPC domain, which defines nuclear localisation and eventually enables oligomerisation. In terms of phylogenetics, the AHL proteins are divided into clades A and B on the basis of number and type of these domains. When it comes to affecting plant development, these nuclear proteins are capable of bonding with the corresponding DNA, and in cooperation with other factors influencing gene expression. In the plant body the AHL proteins are involved in root and floral organogenesis and also cooperate with a number of signaling pathways of phytohormones, such as auxins, gibberellins, brassinosteroids or senescence hormones. Moreover, they take part in photomorphogenesis or control systematic immune responses of the plant. Keywords: AHL, AT-hook, PPC domain, nuclear protein, Arabidopsis thaliana
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Closer analysis of gene trap line MGT180 and its candidate genes
Šnajdrová, Tereza ; Soukup, Aleš (advisor) ; Klíma, Petr (referee)
Root branching allows plants to explore rhizosphere, to gain efficiently water, mineral nutrients or enter in various biotic interactions. Initiation of lateral root formation is localized to pericycle cells, flanking the xylem poles of diarch vascular bundle in Arabidopsis thaliana. Right in these pericycle cells, there is the expression pattern of the gene trap line MGT180. In this theseis , I have provided the evidence that the expression pattern of MGT180 is related with AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED18 (AHL18; At3g60870). AHL18 belongs to a gene family of 29 transcriptional factors of Arabidopsis. AHL18 has not been functionally characterized yet, the analysis of singlemutant ahl18 and some others revealed no significant phenotype. However, one of doublemutants, E15, showed a significant phenotype. This phenotype was evident mainly in the aboveground part of plants, and was not corresponding to any phenotype of AHL mutation described so far. There is a known redundancy among some AHL genes, confirmed by crossing of ahl18 and ahl28 leading to E15 plant. Translation phusion AHL18-mRUBY and AHL22-mRUBY under native promotors should reveal, where these two related proteins act, and if they fiction in autonomous manner or not. Key words Arabidopsis thaliana, lateral root, AHL, pericycle
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AHL genes in plant development
Škrabálková, Eliška ; Širl, Marek (advisor) ; Ortmannová, Jitka (referee)
Plant development is a complex process where different factors come into play. The expression of certain genes, which determine basic plant structure and its signaling pathways, is also of great importance. The AHL gene family, which in case of Arabidopsis thaliana includes 29 members, is one of those determinators that have an impact on plant development on several levels. As far as structure is concerned, the AHL proteins are typically composed of two parts - the DNA-bonding AT-hook motif and PPC domain, which defines nuclear localisation and eventually enables oligomerisation. In terms of phylogenetics, the AHL proteins are divided into clades A and B on the basis of number and type of these domains. When it comes to affecting plant development, these nuclear proteins are capable of bonding with the corresponding DNA, and in cooperation with other factors influencing gene expression. In the plant body the AHL proteins are involved in root and floral organogenesis and also cooperate with a number of signaling pathways of phytohormones, such as auxins, gibberellins, brassinosteroids or senescence hormones. Moreover, they take part in photomorphogenesis or control systematic immune responses of the plant. Keywords: AHL, AT-hook, PPC domain, nuclear protein, Arabidopsis thaliana
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Closer analysis of gene trap line MGT180 and its candidate genes
Šnajdrová, Tereza ; Soukup, Aleš (advisor) ; Klíma, Petr (referee)
Root branching allows plants to explore rhizosphere, to gain efficiently water, mineral nutrients or enter in various biotic interactions. Initiation of lateral root formation is localized to pericycle cells, flanking the xylem poles of diarch vascular bundle in Arabidopsis thaliana. Right in these pericycle cells, there is the expression pattern of the gene trap line MGT180. In this theseis , I have provided the evidence that the expression pattern of MGT180 is related with AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED18 (AHL18; At3g60870). AHL18 belongs to a gene family of 29 transcriptional factors of Arabidopsis. AHL18 has not been functionally characterized yet, the analysis of singlemutant ahl18 and some others revealed no significant phenotype. However, one of doublemutants, E15, showed a significant phenotype. This phenotype was evident mainly in the aboveground part of plants, and was not corresponding to any phenotype of AHL mutation described so far. There is a known redundancy among some AHL genes, confirmed by crossing of ahl18 and ahl28 leading to E15 plant. Translation phusion AHL18-mRUBY and AHL22-mRUBY under native promotors should reveal, where these two related proteins act, and if they fiction in autonomous manner or not. Key words Arabidopsis thaliana, lateral root, AHL, pericycle
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