|
|
Electrochemical properties of selected psychoactive compounds
Jiroušková, Eliška ; Sokolová, Romana (advisor) ; Fischer, Jan (referee)
This Diploma Thesis focuses on the electrochemistry and spectroelectrochemistry of three new psychoactive substances (NPS). These compounds are easily accessible on the market and they are not regulated by current legislation. Due to the huge amount of these drugs it is difficult to identify them in human organism. Three representatives of new psychoactive substances were studied: two stimulants (3-fluorophenmetrazine, 3-FPM and 4-methylpentedrone, 4-MPD) and one of the fentanyl derivatives (FMAcF, m-fluoro methoxyaceyl fentanyl). Drugs often undergo oxidation or reduction during biotransformation in human organism therefore the electrochemical behaviour was studied. The main goal of this work was to determine the initial intermediates of redox processes, clarify reaction scheme (including electron transfer coupled with chemical reaction) and suggest the redox mechanism. The electrochemical properties were studied using cyclic voltammetry with a glassy carbon electrode as a working electrode. The compounds 3-FPM and FMAcF were oxidized within the available potential window and 4-MPD was both reduced and oxidized. The reaction scheme of all compounds was found to be ECE. UV/Vis spectroelectrochemistry revealed the formation of reaction intermediates. Infrared spectroelectrochemistry and...
|
|
|
Electrochemical properties of synthetic drugs 3-fluorophenmetrazine and 4-methylpentedrone
Jiroušková, Eliška ; Schwarzová, Karolina (advisor) ; Dejmková, Hana (referee)
The aim of this Thesis was to clarify electrochemical properties of two new psychoactive substances. These substances are an alternative to classical drugs because they are not controlled by current legislation. It is important to determine the metabolites of these substances to be able to detect them in organism. Their electrochemical properties could explain the process of the first phase of biotransformation in human organism. Two synthetic stimulants were studied - 3-fluorophenmetrazine and 4-methylpentedrone, used as substitutes for cocaine or ecstasy. The purity of substances was controlled by HPLC with UV/Vis diode array detector and the stability was verified by UV/Vis spectrophotometry. Electrochemical properties were studied in phosphate buffer using cyclic voltammetry. For both substances was calculated theoretical HOMO and LUMO spatial distribution. This information was important to estimate parts of molecules, that can be reduced or oxidised. The analytical method was developed on glassy carbon electrode using differential pulse voltammetry. Substance 3-fluorophenmetrazine was determined in phosphate buffer pH 9,0 with limit of detection of 5,1 µmol l-1 . The linear range of calibration curve is from 7,0 to 107,0 µmol/l, R = 0,9988. 4-methylpentedrone was determined in phosphate...
|
guest ::
