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Analýza průběhu výkrmu býků českého strakatého plemene
BALDÍK, Rudolf
Czech mottled cattle is the original breed of cattle in the Czech Republic. It is part of a worldwide population of mottled breeds of the same phylogenetic origin, widespread, for all its continents, for its excellent properties and widespread use. It currently accounts for about one half of the total cattle population in the Czech Republic. The aim of the work was to monitor the growth of fattened bulls, focusing on the last half year of fattening and trying to determine the inflection point of fattening this breed under given breeding conditions. Furthermore, the monitored bulls were compared on the basis of genetic potential, ie the representation of the blood of the Czech spotted breed and according to the father. Fattening bulls are only from their own breeding as weaners from milked herds. The bulls are fattened up to the age of 24 months and are divided into groups of about 24pcs, depending on the weight. The feed is given to them twice a day, at regular intervals, with a mixing feed wagon with weight. The feed consists of corn silage, clover-grass silage and a core mixture, which the company produces itself. The results obtained did not show a statistically significant difference in the pre-slaughter live weight by father line, by month of birth of the individuals studied or by age at slaughter. Only by comparing the live weight before slaughter by SEUROP, there was a statistically significant difference.
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Genetic polymorphism of selected loci in relationship to technological traits of meat
VERNEROVÁ, Kateřina
Within the framework of this diploma, a genotypization of 162 beef cattle of the ČESTR commercial breed from was performed in order to analyze the potential influence of gene coding calpain I (CAPNI) on the resulting beef tenderness. CAPN1 present on the BTA29 chromosome, was selected in previous studies as a candidate gen for QTL. Polymorphism of CAPNI gene was studied using PCR/RFLP method and CAPN530 marker. Genotype identification resulted from this procedure. A 341 bp long fragment was present in homozygotes AA, two fragments of 195 and 146 bp were present in homozygotes GG, and three fragments of 341, 195 and 146 bp were detected in heterozygotes AG. The genotypization output was subsequently statistically evaluated. 11 homozygotes AA, 62 homozygotes GG and 89 heterozygotes AG were detected in the analyzed beef cattle population. More frequent G allele occurred in the set with the frequency of 0,657 and A allele with the frequency of 0,343. Warner-Bratzler shear test was employed to determine beef tenderness based on the shear force attribute. Samples of raw and grilled beef aging 1, 14 and 28 days after the slaughter were analyzed. Statistical methods were used to evaluate relationship between genotype and detected amount of shear force. In case of raw beef, only the samples aging 1 day after the slaughter showed significant difference in the shear force. The most positive results for the given parameter were achieved in case of AG genotype. Samples aging 14 and 28 days after the slaughter showed no difference in shear force and related beef tenderness, indicating no genotype influence. In case of grilled beef, no significant difference in sheer force indicating possible genotype influence was detected at any day of analysis. Genotype influence on grilled beef tenderness within the test animal population is minimal and statistically inconclusive.
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