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Design of motorized optical system used for focusing of a laser beam
Zobač, Evžen ; Novotný, Karel (referee) ; Novotný, Jan (advisor)
This diploma thesis deals with the structural and optomechanical design of automatization of focusing laser beam for a remote laser spectroscopy device. The chosen solution uses an active rangefinder and a rotary unit to control the change of the focus plane of the device's optic system. The dependence of the focus plane’s change on the shift of lens in the focus plane was practically measured. For proper control of the rotary unit, this dependency is needed. Structural parts were made based on the created drawing documentation. The structural parts were mounted in the existing assembly of the device together with the purchased components. The proposed solution enables a change of the optical system, for example, in order to obtain a more appropriate range of focal planes.
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Applications of Fluorescence Holographic Microscope
Zobač, Evžen ; Dostál, Zbyněk (referee) ; Kolman, Pavel (advisor)
This bacherol thesis discusses the holographic fluorescence microscopy, interferometric microscope with off-axis interference. It is divided into a theoretical part and an experimental part. The theoretical part describes the setting of the holographic fluorescent microscope and basic theory of fluorescence interferometry. There are also described the methods used to increase the resolution of fluorescence microscopy. The practical part deals with the verification of the imaging properties of holographic fluorescence microscope and opportunities for enhanced imaging features and measurements.
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Design of motorized optical system used for focusing of a laser beam
Zobač, Evžen ; Novotný, Karel (referee) ; Novotný, Jan (advisor)
This diploma thesis deals with the structural and optomechanical design of automatization of focusing laser beam for a remote laser spectroscopy device. The chosen solution uses an active rangefinder and a rotary unit to control the change of the focus plane of the device's optic system. The dependence of the focus plane’s change on the shift of lens in the focus plane was practically measured. For proper control of the rotary unit, this dependency is needed. Structural parts were made based on the created drawing documentation. The structural parts were mounted in the existing assembly of the device together with the purchased components. The proposed solution enables a change of the optical system, for example, in order to obtain a more appropriate range of focal planes.
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Applications of Fluorescence Holographic Microscope
Zobač, Evžen ; Dostál, Zbyněk (referee) ; Kolman, Pavel (advisor)
This bacherol thesis discusses the holographic fluorescence microscopy, interferometric microscope with off-axis interference. It is divided into a theoretical part and an experimental part. The theoretical part describes the setting of the holographic fluorescent microscope and basic theory of fluorescence interferometry. There are also described the methods used to increase the resolution of fluorescence microscopy. The practical part deals with the verification of the imaging properties of holographic fluorescence microscope and opportunities for enhanced imaging features and measurements.
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