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Investigations of uratic concrements in urolithiasis
Kultová, Lenka ; Zadina, Jiří (advisor) ; Kotaška, Karel (referee)
Urolithiasis is wide - spread disease, which can lead to serious complications as far as acute renal failure. It is important to analyze the composition of calculus for accurate diagnosis and effective prevention. I deal with analysis of urinary stones by infrared spectroscopy and polarization microscopy in this work. In the first part of the work I intent on the methodical aspects of assesment. The set of ten samples, which included rife components of calculi, was evaluated by two software programs - Kidney Stone Analysis and Omnic. Programme Kidney Stone Analysis gives just one end - result. Programme Omnic indicates ten possible results of the constitution of the sample, which best matches with spectra from libraries. The evaluative person has a possibility to compare these results and decide for the end - result, according to how quantitative data in the evaluation repeat. The result of component's content around 10 % can not be regard as totally reliable. The polarization microscope enables to check and specify the results of the analysis. To use a combination of both methods claimed to be very profitable. In the second part of the work, patients' urinary stones were analyzed, in the way how samples are evaluated in laboratory running. Patient group was formed by 20 persons (17 men and 3 women) in the...
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Significance of determination SAA of comparison with CRP and procalcitonin
Daňková, Michaela ; Zadina, Jiří (advisor) ; Průša, Richard (referee)
The purpose of the bachelor thesis is to evaluate serum amyloid (SAA) as a diagnostic marker in selected patient groups and to compare the results of SAA with those of C-reactive protein (CRP) and procalcitonin (PCT). SAA, CRP and PCT are acute-phase proteins, whose blood concentration significantly increases within a few hours after an inflammatory stimulus. The synthesis, caused by proinflammatory cytokines, takes place in the liver. CRP is the most and longest used acute-phase reactant in routine practice. As indicators of inflammation, SAA and especially procalcitonin have been used more lately. Although SAA is an acute-phase reactant as sensitive as CRP, it is not employed so often in the diagnosis since a method suitable for routine diagnosis was not developed until recently. Briefly describing the acute-phase proteins, the theoretical part elaborates on the characterization and comparison of SAA, CRP and PCT. It further outlines methods for the determination of individual analytes. The experimental part focuses on defining selected patient groups and the immunonephelometric method for measuring SAA by the Immage 800 biochemical analyzer. The data obtained in three selected patient groups have been statistically evaluated. The aim of the thesis has been to evaluate the correlation of inflammatory...
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The posibility of inorganic sulphate determination in patients with severe metabolic disease
Plasová, Martina ; Kotaška, Karel (advisor) ; Zadina, Jiří (referee)
Inorganic Sulphate play important role in various metabolic pathways. The aim of the work was to assess the posibility of inorganic sulphate determination in patients with severe kidney diseses undergoing hemodialysis. Serum sulphate levels were determined in 68 patients (31 men, mean age 67, range 31 - 86 and 37 women mean age 71, range 44 - 86) before and after hemodialysis. Two turbidimetric methods Quantichrom TM Sulfate Assay Kit and BGR gelatine method were used for determination. Both methods were performed separately in group of 34 patients. Results were as follows: Mean serum sulphate levels in patients before hemodialysis are significantly elevated than the levels after hemodialysis (p<0.0001, non parametric t-test, alpha = 0.05). BGR gelatine method show less significant differences in sulphate levels before and after hemodialysis than the Quantichrome Assay method. (p = 0.037 vs. p < 0.001, non parametric t-test, alpha = 0.05 ). This fact could be explained by the significant role of interferences in BGR gelatine method. We confirm the relevance of both metod in inorganic sulphate determination. BGR gelatine method is more affected by interference, than the QuantiChrome Assay method. Powered by TCPDF (www.tcpdf.org)
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Significance of determination SAA of comparison with CRP and procalcitonin
Daňková, Michaela ; Zadina, Jiří (advisor) ; Průša, Richard (referee)
The purpose of the bachelor thesis is to evaluate serum amyloid (SAA) as a diagnostic marker in selected patient groups and to compare the results of SAA with those of C-reactive protein (CRP) and procalcitonin (PCT). SAA, CRP and PCT are acute-phase proteins, whose blood concentration significantly increases within a few hours after an inflammatory stimulus. The synthesis, caused by proinflammatory cytokines, takes place in the liver. CRP is the most and longest used acute-phase reactant in routine practice. As indicators of inflammation, SAA and especially procalcitonin have been used more lately. Although SAA is an acute-phase reactant as sensitive as CRP, it is not employed so often in the diagnosis since a method suitable for routine diagnosis was not developed until recently. Briefly describing the acute-phase proteins, the theoretical part elaborates on the characterization and comparison of SAA, CRP and PCT. It further outlines methods for the determination of individual analytes. The experimental part focuses on defining selected patient groups and the immunonephelometric method for measuring SAA by the Immage 800 biochemical analyzer. The data obtained in three selected patient groups have been statistically evaluated. The aim of the thesis has been to evaluate the correlation of inflammatory...
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The posibility of inorganic sulphate determination in patients with severe metabolic disease
Plasová, Martina ; Kotaška, Karel (advisor) ; Zadina, Jiří (referee)
Inorganic Sulphate play important role in various metabolic pathways. The aim of the work was to assess the posibility of inorganic sulphate determination in patients with severe kidney diseses undergoing hemodialysis. Serum sulphate levels were determined in 68 patients (31 men, mean age 67, range 31 - 86 and 37 women mean age 71, range 44 - 86) before and after hemodialysis. Two turbidimetric methods Quantichrom TM Sulfate Assay Kit and BGR gelatine method were used for determination. Both methods were performed separately in group of 34 patients. Results were as follows: Mean serum sulphate levels in patients before hemodialysis are significantly elevated than the levels after hemodialysis (p<0.0001, non parametric t-test, alpha = 0.05). BGR gelatine method show less significant differences in sulphate levels before and after hemodialysis than the Quantichrome Assay method. (p = 0.037 vs. p < 0.001, non parametric t-test, alpha = 0.05 ). This fact could be explained by the significant role of interferences in BGR gelatine method. We confirm the relevance of both metod in inorganic sulphate determination. BGR gelatine method is more affected by interference, than the QuantiChrome Assay method. Powered by TCPDF (www.tcpdf.org)
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Investigations of uratic concrements in urolithiasis
Kultová, Lenka ; Zadina, Jiří (advisor) ; Kotaška, Karel (referee)
Urolithiasis is wide - spread disease, which can lead to serious complications as far as acute renal failure. It is important to analyze the composition of calculus for accurate diagnosis and effective prevention. I deal with analysis of urinary stones by infrared spectroscopy and polarization microscopy in this work. In the first part of the work I intent on the methodical aspects of assesment. The set of ten samples, which included rife components of calculi, was evaluated by two software programs - Kidney Stone Analysis and Omnic. Programme Kidney Stone Analysis gives just one end - result. Programme Omnic indicates ten possible results of the constitution of the sample, which best matches with spectra from libraries. The evaluative person has a possibility to compare these results and decide for the end - result, according to how quantitative data in the evaluation repeat. The result of component's content around 10 % can not be regard as totally reliable. The polarization microscope enables to check and specify the results of the analysis. To use a combination of both methods claimed to be very profitable. In the second part of the work, patients' urinary stones were analyzed, in the way how samples are evaluated in laboratory running. Patient group was formed by 20 persons (17 men and 3 women) in the...
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