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Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method II.
Stanciová, Cristina ; Vejsová, Marcela (advisor) ; Křivčíková, Lucie (referee)
5 Abstract Cristina Stanciová Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method II Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods The aim of this thesis was to test substances produced by the Department of Pharmaceutical Chemistry and Drug Control and the Department of Inorganic and Organic Chemistry Faculty of Pharmacy of Charles University in Hradec Králové. Substances were tested by using microdilution broth method. Testing was carried out on eight strains of the bacteria: Staphylococcus aureus, Staphylococcus aureus methicilin resistant, Staphylococcus epidermidis, Enterococcus sp., Escherichia coli, Klebsiella pneumoniae and Pseudoonas aeruginosa. Overall were tested 97 substances, which were divided into ten groups according to their chemical structure to: derivatives of (Z)-5- arylmethyliden-2-thioxothiazolidin- 4-on, anilids of pyrazinocarboxyl acid, arylaminopyrazins, pyrazin-2,3-dikarbonitrils, esters of pyrazincarboxyl acid, N- (2-anilino-1-alkyl-2-oxo-ethyl)-2-hydroxy-benzamides, esters of cholesterol and alkan΄s acids, salicylthioamids, styryl benzoxazols and benzoxazepin-dions. The most effective was group of salicylthioamid derivates. Substances T5619 and T5621 took...
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Molecular diagnosis of viral hepatitis C - personal experience from routine laboratory
Horáčková, Veronika ; Vejsová, Marcela (advisor) ; Fajfr, Miroslav (referee)
Author: Veronika Horáčková Title: Molecular diagnosis of viral hepatitis C - personal experience from routine laboratory Bachler work Field of study: Medical laboratory technician, combined study form The aim of the study: Introduction to the issues concerning the viral Hepatitis C with an emphasis on the laboratory diagnostics thereof. Statistical processing of the Hepatitis C virus genotype analysis results. Material and methods: In the period of 1 July 2005 - 30 June 2012, the Hepatitis C virus genotype analysis was performed in respect of 2 185 samples at the division of molecular biological diagnostics of the serology laboratory of the Institute of Clinical Biochemistry and Laboratory Diagnostics of the General Faculty Hospital and the First Faculty of Medicine of Charles University in Prague, using the reverse hybridization method. The testing results were statistically processed. Results: Of the set of 2 185 samples, 1 372 (62,8 %) originated from males and 813 (37,2 %) from females, with the average age of patients included in the set being 36,7 years. The majority of requests for the HCV genotype analysis originated from specialists in Prague (91,0 %). The genotype analysis demonstrated the highest prevalence of the 1b genotype (51,6 %), followed by the 3a genotype (22,6 %). The prevalence...
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Evaluation of activity of potentional antifungal substances through the use of microdilution broth method I.
Štvrtecká, Jana ; Vejsová, Marcela (advisor) ; Paterová, Pavla (referee)
Jana Štvrtecká Evaluation of activity of potentional antifungal substances through the use of microdilution broth method I Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralové Medical bioanalytic_____________________________________________________ The aim of this thesis is to evaluate the potencial in vitro antifungal activity of selected drugs synthetised on the Department of Pharmaceutical Chemistry and Drug Control and on the Department of Anorganic and Organic Chemismy on the Faculty of Pharmacy in Hradec králové. For drug testing was used the microdilution broth method. The tested compounds were tested on followed five yeast strains and three filamentous fungi models which are pathogenic for humans: Candida albicans ATCC 44859, Candida tropicalis 156, Candida krusei E28, Candida glabrata 20/I, Trichosporon beigelii 1188, Aspergillus fumigatus 231, Absidia corymbifera 272, Trichophyton mentagrophytes 445. 80 compounds were divided into 10 groups accorging to their chemical structures: anilides of pyrazin carboxyl acids, arylamin pyrazines, pyrazin carboxyl acid esters, pyrazine-2,3-dinitriles, benzoxazepin-diones, diamides, styryl benzoxazoles, derivatives of (Z)-5-arylmethyliden-2-thioxothiazolidin-4-on, ester derivatives of cholesterol and alcan acids and...
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In situ analýzy zánětlivých kolitid Muc2-/- myší.
Drábková, Martina ; Vejsová, Marcela (advisor) ; Jílek, Petr (referee)
Author: Martina Drábková Title: In situ analyses of inflammatory colitis in Muc2-/- mice Diploma thesis Charles University in Prague, Faculty of pharmacy in Hradec Králové Pharmacy Background: Muc2 mucin is the main structural component of the intestinal mucus which forms important protective barrier avoiding the direct contact between the commensal bacteria and the epithelium. In order to understand the role of Muc2 mucin a Muc2 deficient mice strain has been developed. Initial observation showed that these mice already in few weeks after the birth develop spontaneous colitis and later on colon cancer. To better understanding what is going on in the colon and small intestine of Muc2-/- mice, in this thesis we focused on in situ analyses of distribution of different immune cells in these tissues. Methods: In our experiments three Muc2-/- , two Muc2+/- and three wild type mice on the C57BL/6 background were used. For the detection of distribution of different immune cells within lamina propria of small intestine and colon the immunohistochemistry analyses on sections from these tissues were performed. Results: In the lamina propria of distal colon of Muc2-/- mice we found an increase of neutrophils followed by increase of macrophages, CD4+ T cells and B cells/plasma cells compared to control and...
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Candida species
Drbohlavová, Anna ; Křivčíková, Lucie (advisor) ; Vejsová, Marcela (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Anna Drbohlavová Supervisor: Ing. Lucie Křivčíková Title of the bachelor thesis: Candida species This bachelor thesis deals with a laboratory diagnosis of Candida spp. The main goal is to create a compact survey of laboratory methods which are utilised for a laboratory diagnosis of Candida species especially in the microbiological laboratory. The thesis is divided into four parts. The first part focuses on the common characteristic of the kingdom Fungi and the yeasts. The second part serves as the basic laboratory methods survey for a laboratory diagnosis of Candida spp. The primary classification of mycotic diseases with their basic characterisation is processed in the third part. Furthermore, this part includes the most frequent agents of these diseases from the genus Candida as well and the ways of clinical material taking. The fourth part is devoted to the characterisation of the most frequent Candida spp. agents of the yeast infections. The whole thesis is completed with a photographic appendix of selected Candida species. Key words: Candida spp., laboratory diagnosis, yeast infections
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Human papillomavirus vaccination
Dvořáková, Iveta ; Vejsová, Marcela (advisor) ; Fajfr, Miroslav (referee)
Author: Bc. Dvořáková Iveta Title: Human papillomavirus vaccination Diploma thesis Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové Field study: Specialist in laboratory methods Background: The aim of this work is to bring general awareness of cervical cancer, its creator, transmission, symptoms and treatment. It presents information about registered vaccines on the market. The aim of this study is to determine women and girls awareness of vaccination against cervical cancer, and their willingness to be vaccinated. Determine view - gynecologist vaccination against cervical cancer. Main findings: Vaccination against cervical cancer is already included in the vaccination calendar. Not an obligatory vaccination but only as recommended vaccinations for girls between 13 and 14 years. Conclusion: The diploma paper indicates the general awareness of the causative agent, transfer options and the prevention of cervical cancer among girls. The study found that most doctors inform girls and women about the possibility to be vaccinated. Doctors would recommend vaccination for men too. Most mothers are trying to have regular preventive examinations. The study found that most mothers are going to vaccinate their daughters, but they would not get vaccinated. Key words: Cervical cancer, Human...
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Current methods in diagnostics, treatment and prevention of infection caused by methicilin resistant Staphylococcus aureus (MRSA)
Korejsová, Erika ; Konečná, Klára (advisor) ; Vejsová, Marcela (referee)
Candidate: Erika Korejsová Supervisor: Mgr. Klára Konečná, Ph.D. Consultant: MUDr. Marie Smolíková Title: Current methods in diagnostics, treatment and prevention of infection caused by methicilin resistant Staphylococcus aureus (MRSA) Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study field: Medical Bioanalytics Staphylococcus aureus is a bacterium that is found in the nasal mucosa of healthy people (20-40%) and skin without causing disease. Under certain circumstances, such as damage or injury of skin, mucous membranes and in immuno-incompetent individuals, the MRSA infection can originate. Therefore, hospitalized patients are much more susceptible to this infection. The biggest dangers represent MRSA (methicillin-resistant Staphylococcus aureus) strains. These strains of Staphylococcus aureus are mostly multi-resistant, where in addition to resistance to oxacillin (methicillin), resistence to other antibiotik occurs as well. Treatment of these patients is problematic, because MRSA strains are extensively resistant and persist in the organism and in the environment. Their eradication is not simple. The patient's blood reinfection often occurs due to low levels of antibiotics. It means a considerable financial...
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