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Biotechnological potential of algae and cyanobacteria from low temperature environments
Faško, Adam ; Nedbalová, Linda (advisor) ; Vítová, Milada (referee)
With the interest in biofuels rising, public awareness of the biotechnological potential of algae and cyanobacteria has grown and there is discussion about other possible uses of these microorganisms. Algae and cyanobacteria from low temperature environments possess exceptional properties as part of their adaptation strategies. The aim of this work is to evaluate whether these properties can be exploited in biotechnology. In my theses, I focus on the three most frequently discussed areas of application of algae and cyanobacteria from cold environments: production of polyunsaturated fatty acids, dyes and antioxidants, and wastewater treatment. Based on the available literature, I concluded that algae and cyanobacteria from low temperature environments can outperform mesophilic species in terms of production of desired substances or nutrient uptake from the medium under certain conditions. Selection of suitable strains, optimization of culture conditions and application of metabolic engineering and synthetic biology will have a major impact on the utilization rate of these microorganisms.
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Cirkadiální hodiny se neúčastní načasování buněčného dělení v řase Chlamydomonas reinhardtii
Vítová, Milada ; Bišová, Kateřina ; Hendrychová, Jana ; Čížková, Mária ; Zachleder, Vilém
To determine whether the circadian clock functions in control the entry of algal cells into the cell cycle, we have tested the green alga Chlamydomonas reinhardtii in a wide range of growth conditions both light intensity and temperature. We monitored the course of the cell cycles in asynchronous as well as in synchronized cultures. The cultures were grown either at the continuous light or at alternating light/dark periods. The length of the cell cycle was monitored also at different temperatures and in “circadian” mutant. We have evidenced that the length of the cell cycle corresponding to circadian times can be attained only under relatively narrow range of growth conditions and even in this case the “circadian time” is a result of given growth conditions and not of any circadian gating. These results suggest that the intrinsic circadian clock does not play any role in timing of the cell cycle division in the green alga Chlamydomonas
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Plastoskeletální protein FtsZ a dělení chloroplastů v zelených řasách
Vítová, Milada ; Hendrychová, Jana ; Bišová, Kateřina
FtsZ protein, an ancestral homologue of eukaryotic tubulin, is the key effector in regulation of bacterial division. In plants, the FtsZ protein participates in chloroplast division. We have studied the chloroplast division in the green unicellular alga Scenedesmus quadricauda. To uncouple chloroplast and nucleocytoplasmic division cycles, we used 5-fluorodeooxyuridine (FdUrd). The amount and localization of FtsZ during the cell cycle was followed using immunoblotting and immunofluorescence staining on semi-thin sections. Antibody against FtsZ reacts with 49kD protein in Scenedesmus quadricauda and this protein is localized in chloroplast.The level of FtsZ protein increases during growth phase with maximum in time of chloroplast division. Localization of FtsZ protein is changing during the cell cycle, but we have not observed Z-ring, described in higher plants
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