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Electronic Shop
Morcinek, Petr ; Stoklasa, Michal (referee) ; Kříž, Jiří (advisor)
This paper focuses on the electronic trade in the Czech Republic and on its current state. Goal of the thesis is to formulate the strategy for employing and operation of the electronic shop which would be understandable for the wide public and create a concrete proposal of the electronic shop including the strategy for the submitter. Theoretical part of the thesis focuses on defining the electronic shop as a concept. There is also an analysis of the current state in the Czech Republic there. Empirical part then formulates the strategy for implementation and operation of the electronic shop and makes a proposal of the strategy for the submitter.
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Mitochondrion-related organelles in diplomonads
Stoklasa, Michal ; Tachezy, Jan (advisor) ; Kolísko, Martin (referee)
Order Diplomonadida includes parasitic and free-living species that adapted to the oxygen-poor environment. They possess reduced form of mitochondria (hydrogenosome or mitosome). These organelles lack Krebs cycle and membrane electron-transport chain. ATP synthesis by oxidative phosphorylation and other mitochondrial metabolic pathways are modified or entirely absent. Main difference between hydrogenosome and mitosome is synthesis of hydrogen using the enzyme hydrogenase and ATP synthesis by substrate level phosphorylation in hydrogenosomes that are absent in mitosomes. The most studied diplomonads are a human pathogen Giardia intestinalis possessing the mitosomes and a salmon parasite Spironucleus salmonicida with hydrogenosomes. This thesis was focused on determining the type of mitochondrial organelles in angelfish parasite Spironucleus vortens and free living Hexamita sp. It has not been described whether they possess the hydrogenosomes or the mitosomes so far. In both protists transmission electron microscopy revealed presence of double membrane vesicles, possibly their mitochondrial organelles. Homologous S. vortens anti- hydrogenase and anti-HydE antibodies were produced and tested in order to determine their cellular localization. Using the western blot analysis and immunofluorescence...
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Mitochondria of anaerobic protists
Stoklasa, Michal ; Tachezy, Jan (advisor) ; Rada, Petr (referee)
Mitochondria are organelles of endosymbiotic origin, known as the energy center of every eukaryotic cell. Main function is the production of adenosin triphosphate (ATP), which is used as an energy source for most metabolic reactions occuring in the cell. Furthermore, mitochondria play significant role in apoptosis and we can find here a number of biochemical pathways such as β-oxidation of fatty acids, synthesis of iron- sulfur clusters, heme, steroids etc. Most of the mitochondrial metabolic functions have been lost during the adaptation to oxygen-poor environment in some of the parasitic (Giardia intestinalis, Entamoeba histolytica) or free-living protists (Sawyeria marylandensis, Mastigamoeba balamuthi). These organisms possess reduced mitochondrial organelles such as hydrogenosome or mitosome. The aim of this thesis is to summarize the information about these reduced mitochondrial organelles of anaerobic protists and to describe their specific metabolism. Key words: mitochondrion, hydrogenosome, mitosome
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Electronic Shop
Morcinek, Petr ; Stoklasa, Michal (referee) ; Kříž, Jiří (advisor)
This paper focuses on the electronic trade in the Czech Republic and on its current state. Goal of the thesis is to formulate the strategy for employing and operation of the electronic shop which would be understandable for the wide public and create a concrete proposal of the electronic shop including the strategy for the submitter. Theoretical part of the thesis focuses on defining the electronic shop as a concept. There is also an analysis of the current state in the Czech Republic there. Empirical part then formulates the strategy for implementation and operation of the electronic shop and makes a proposal of the strategy for the submitter.
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