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The Influence of Selected Environmental Pollutants on Gametogenesis and Early Embryonic Development
Fenclová, Tereza ; Nevoral, Jan (advisor) ; Děd, Lukáš (referee) ; Sovadinová, Iva (referee)
The influence of selected environmental pollutants on gametogenesis and early embryonic development Ing. Tereza Fenclová Idiopatic infertility is a serious problem, which can be caused by exposure to endocrine disruptors, e.g. bisphenols. Bisphenol A, the most widely used bisphenol, is now being replaced in manufacturing processes due to its toxicity by its analogues, bisphenol S and F. While most of the scientific experiments examine the effect of toxic doses after direct exposure, this study provides results for exposure to real low doses after direct and indirect exposure. We assumed a negative effect of direct and indirect exposure to low doses of alternative bisphenols BPS and BPF on male and female reproduction at the level of gametes and embryos. The aim of this work is to evaluate the effect of direct exposure to mouse oocytes and sperm, to evaluate the effect of indirect exposure through breast milk to mouse oocytes, sperm, testicular tissue and the extent of damage to early embryonic development. Experimental ICR laboratory mice were exposed to low doses of bisphenols directly via drinking water or an oral gavage, or indirectly through the breast milk. Direct exposure to low doses of bisphenols affects the reproductive abilities of females mainly by malformations of the dividing spindle of...
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