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Dendritic cells and their importance in immune response
Vávrová, Jitka ; Rožková, Daniela (advisor) ; Bartůňková, Jiřina (referee)
Dendritic cell (DC) is the most effective antigen presenting cell in human organism. Dendritic cells plays important role in immunity response because they stimulate naive T-lymfocytes. This propeliy is very useful in immunotherapy of tumor diseases. The general aim of this work was to optimalize operating protocol for preparation vaccine containing dendritic cell s for patients with ovarian carcinoma. We examined single parts of process of preparation and their impact on result of whole process. We were interested in used plastic, from which are culture bottle made, and its impact to maturation of dendritic cells. Next thing that we examined was if we use the same kind of maturation reagent, type poly I:C, from different producers, will be dendritic cells maturated in the same way. After made vaccines are freezed to -80DC to keep their immunosupresive properties. We examined if DCs after defrosting have the same properties like before. We also examined diferent types of medium used for freezing and their impact to fenotype and service life ofDCs. Powered by TCPDF (www.tcpdf.org)
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Immunotherapy of ovarian carcinoma with dendritic cells
Partlová, Simona ; Rožková, Daniela (advisor) ; Froňková, Eva (referee)
V ANGLICKÉM JAZYCE Immunotherapy of ovarian carcinoma with dendritic cells Anticancer immunotherapy is a therapeutical strategy aimed at elicitation and maintenance of immune responses against cancer cells. In this study we have focused on immunotherapy of ovarian cancer, because it is one of the most common gynaecological tumors with poor prognosis and high mortality. Our immunotherapy protocol involves preparing dendritic cells (DC) from monocytes isolated from patient's peripheral blood, which are subsequently pulsed with irradiated cells of established ovarian cancer cell line. These immature pulsed DC are maturated and subsequently co-cultivated with autologous T lymphocytes. The aim of this study was to demonstrate, that DC are able to elicit specific immune response after addition of suitable mature agens in combination with apoptotic ovarian tumor cells. Our observations indicate that 24 hours are sufficient for induction of tumor cells apoptosis. Additionally, we have shown that DC successfully ingested most of the apoptotic tumor cells after 4 hours of co-incubation. Furthermore, we have found out that ingestion of apoptotic cells by dendritic cells, which are stimulated with polyI:C, inhibits maturation of DC and consequently also production of cytokines IL-12p70, IL-6 and TNF-α. Whereas...
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Dendritic cells and their importance in immune response
Vávrová, Jitka ; Rožková, Daniela (advisor) ; Bartůňková, Jiřina (referee)
Dendritic cell (DC) is the most effective antigen presenting cell in human organism. Dendritic cells plays important role in immunity response because they stimulate naive T-lymfocytes. This propeliy is very useful in immunotherapy of tumor diseases. The general aim of this work was to optimalize operating protocol for preparation vaccine containing dendritic cell s for patients with ovarian carcinoma. We examined single parts of process of preparation and their impact on result of whole process. We were interested in used plastic, from which are culture bottle made, and its impact to maturation of dendritic cells. Next thing that we examined was if we use the same kind of maturation reagent, type poly I:C, from different producers, will be dendritic cells maturated in the same way. After made vaccines are freezed to -80DC to keep their immunosupresive properties. We examined if DCs after defrosting have the same properties like before. We also examined diferent types of medium used for freezing and their impact to fenotype and service life ofDCs. Powered by TCPDF (www.tcpdf.org)
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