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Effect of morphine application on the rat circadian system
Platilová, Michaela ; Bendová, Zdeňka (advisor) ; Svobodová, Irena (referee)
Circadian rhythms are controlled by endogenous mammalian biological oscillators. The main oscillator is located in the suprachiasmatic nuclei in the hypothalamus and entrains the phases of slave peripheral oscillators located in many organs and tissues. Under natural conditions, circadian rhythms are synchronized with an external 24-hour day, by light-dark changes during the day. In addition, the circadian system is sensitive to a number of non-photic stimuli, such as arousal or social interactions, severe stress, medicines, alcohol or opiates. Timed application of opioids causes phase shifts of the circadian rhythmicity in behaviour and negatively affects light synchronization. The aim of this work is to explore the effect of acute morphine on the activity of cellular signaling cascades in SCN cells, that are essential for the proper function of the circadian clock, and to elucidate the molecular interaction between opioid and circadian system. Our data suggest that acute activation of opioid signalization affect the circadian clock indirectly, and suprachiasmatic nuclei obtain the information via afferents form other part of brain. Besides that, the side data of our work show the decrease of pERK1/2 a pGSK3β within two hours after the light pulse, which is the observation that has not been yet...
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