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Influence of surfactants and cosmetic polysaccharides on skin parameters and human skin microbiome
Pilipenco, Alina ; Hurčíková, Andrea (referee) ; Ilona,, Matějková (advisor)
The aim of this diploma thesis was to investigate the effect of surfactants and cosmetic polysaccharides on skin parameters and its microbiome. Three surfactants were tested to determine their effect: Sodium Dodecyl Sulfate (SDS), Cocamidopropyl Betaine (CAPB), Decylglucoside (DG). Distilled water was also used for comparison. For the next part of the experimental work were selected 6 polysaccharides: high molecular weight Hyaluronic Acid (HMW HA), very low molecular weight Hyaluronic Acid (VLMW HA), Sodium Caproyl Hyaluronate (CaproylHA), Sodium Carboxymethyl -Glucan (NaCMG), Schizophyllan and Glucomannan. For comparison, placebo and untreated control (only CAPB treatment) were also included in the tests. The first part of the work is a literature search on the assigned topic, which contains the following parts: skin anatomy and its biophysical properties, skin microbiome and its functions, description of used surfactants and polysaccharides. The experimental part is mainly focused on bioengineering methods for evaluation of skin parameters and qRT-PCR to determine the relative proportion of main bacterial species of skin microbiome. First, the effect on the CT gene of 16S rDNA was analysed, and Propionibacterium acnes and Staphylococcus epidermidis strains were selected for further analysis. In conclusion are presented an overview of all properties of selected substances and assessment of their application in cosmetics.
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Use of rare oils in cosmetics
Pilipenco, Alina ; Szotkowski, Martin (referee) ; Němcová, Andrea (advisor)
The purpose of this bachelor thesis was to research rare oils. The oils from selected plants (poppy, cannabis, thistle) is characterized by the content of active phenolic compounds, fatty acid composition, antioxidant and antimicrobial effects. First part of the thesis is a literary research on a given topic, which contains the following parts: the universal chemical composition of vegetable oils, the characteristics and effects of selected oils and the ways of their obtaining for cosmetic purposes. The experimental part is focused primarily on the basic analysis of selected oil components and the preparation of the shower oil based on the obtained results. In conclusion are presented an overview of all the properties of selected oils, their practical use and assessment of their application in cosmetics.
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Influence of surfactants and cosmetic polysaccharides on skin parameters and human skin microbiome
Pilipenco, Alina ; Hurčíková, Andrea (referee) ; Ilona,, Matějková (advisor)
The aim of this diploma thesis was to investigate the effect of surfactants and cosmetic polysaccharides on skin parameters and its microbiome. Three surfactants were tested to determine their effect: Sodium Dodecyl Sulfate (SDS), Cocamidopropyl Betaine (CAPB), Decylglucoside (DG). Distilled water was also used for comparison. For the next part of the experimental work were selected 6 polysaccharides: high molecular weight Hyaluronic Acid (HMW HA), very low molecular weight Hyaluronic Acid (VLMW HA), Sodium Caproyl Hyaluronate (CaproylHA), Sodium Carboxymethyl -Glucan (NaCMG), Schizophyllan and Glucomannan. For comparison, placebo and untreated control (only CAPB treatment) were also included in the tests. The first part of the work is a literature search on the assigned topic, which contains the following parts: skin anatomy and its biophysical properties, skin microbiome and its functions, description of used surfactants and polysaccharides. The experimental part is mainly focused on bioengineering methods for evaluation of skin parameters and qRT-PCR to determine the relative proportion of main bacterial species of skin microbiome. First, the effect on the CT gene of 16S rDNA was analysed, and Propionibacterium acnes and Staphylococcus epidermidis strains were selected for further analysis. In conclusion are presented an overview of all properties of selected substances and assessment of their application in cosmetics.
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Use of rare oils in cosmetics
Pilipenco, Alina ; Szotkowski, Martin (referee) ; Němcová, Andrea (advisor)
The purpose of this bachelor thesis was to research rare oils. The oils from selected plants (poppy, cannabis, thistle) is characterized by the content of active phenolic compounds, fatty acid composition, antioxidant and antimicrobial effects. First part of the thesis is a literary research on a given topic, which contains the following parts: the universal chemical composition of vegetable oils, the characteristics and effects of selected oils and the ways of their obtaining for cosmetic purposes. The experimental part is focused primarily on the basic analysis of selected oil components and the preparation of the shower oil based on the obtained results. In conclusion are presented an overview of all the properties of selected oils, their practical use and assessment of their application in cosmetics.
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