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DOES ONE MONTH CREATINE SUPPLEMENTATION IMPROVE BLOOD LIPIDS?
Kohlíková, E. ; Petr, M. ; Navrátil, Tomáš ; Heyrovský, Michael ; Přistoupilová, K. ; Přistoupil, T. I.
One month’s administration of creatine (CR) in the dose of 5 g/day to 11 young active sportsmen affected their daily amount of CR excreted into urine, and the levels of cholesterol (CH), of triacylglycerols (TAG), of vitamin B12 and of folates in blood. The probands were divided into 4 groups, according to the amount of CR found in urine, and of folates and vitamin B12 determined in blood. It is possible that added CR might induce individually higher offer of 2C units, released under catalysis of vitamin B12 from HoCySH and serine in enzymatic reaction catalyzed by CBS, and in this way higher offer of cysteine and succinyl-CoA This is the main effect of CR in the group D. Both vitamins influence the ratio between glycine (necessary mainly for CR synthesis, beside other metabolic pathways) and serine. The change of serine into glycine can be realized by THF, under catalysis of serine hydroxymethyltransferase. For the opposite change of glycine into serine the metabolic pathway is catalyzed by folates too, but in more complicated way
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The efficiency testing of production commercial feeds for african catfish (Clarias gariepinus)
PETR, Miloš
In my diploma thesis I was tested six types of feed. I tested in 3 repetitions in the recirculating system with biological treatment of water. Tested fishes african catfish (Clarias gariepinus) weighted between 200 ? 800 g per piece, were deployed into 18 aquariums with volume of 190 litres per aquarium. The whole experiment took place in five partial 3-week periods in the intensive breeding of the laboratory - controlled reproduction of fishes FROV JCU. The average water temperature during the experiment was 27 °C. The main objective of the thesis was to observe production efficiency of tested types of feed, speed of the growth of fishes, feed rates and financial costs. Afterwards, determinativ of yield and organoleptic evaluation was determined, followed by laboratory examination of composition of meat. The results of production indicators (Specific Growth Rate, Relative Growth Rate, Food Conversation Ratio) were most favorable for 3 types of feed ? Haltáp (SGR=0,81 %; RGR=126 %; FCR=1,45), Coppens TROCO SUPREME-22 (SGR=0,73 %; RGR=105 %; FCR=1,19), Coppens TROCO PRIME-18 (SGR=0,68 %; RGR=97 %; FCR=1,26). The lowest costs for 1 kg of fish increment were achieved for these specific types of feed Haltáp and Aller Aqua Focus (45 Kč a 46 Kč). In the organoleptic analysis was found positive evaluation of meat of all tested fishes. Chemical composition of meat was (in term of proteins and fats content) most favorably documented for the hungarian feed called Haltáp (proteins=19,22 % and fats=4,85 %).
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Cost calculation in truck transport
Petr, Martin ; Novák, Radek (advisor) ; Šošovička, Lukáš (referee)
Master's Thesis is dealing with cost calculation in truck transport in view of smaller freight and spedition bussines firm which is operating on Czech territory. The main point is to break down cost calculation for all kinds of vehicles, which are owned by analysed firm, relative to the monitored period. Created cost calculation will serve to the employees by price setting for the transport services in future.
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Artificial phospholipid membranes as models of real membranes and transport of charged particles across them
Parisová, Martina ; Navrátil, Tomáš ; Šestáková, Ivana ; Kohlíková, E. ; Petr, M.
Because the real bio-membranes and the tranporting processes realized across them are very complicated, the model membranes have been used for elucidation of the basic transporting steps. In the contribution, the different possibilities of characterization, description, and elucidation of the transport processes on the example of some metal ions and theor complexes, i.e., of cadmium, lead, and copper, were presented. It summarizes the results achieved using supported phospholipid model membranes formed on porous membranes and on agar support. Discussed transporting processes of selected ions have been characterized using two electrochemical methods: electrochemical impendance spectrometry (EIS) and voltammetry. The impact of some parameters on these systems and processes was investigated.
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Evaluation of carp fish-scale reading production indicators in fishponds
PETR, Miloš
The main fish in the Czech pond aquacultures is Cyprinus carpio (common carp). All forms of carp feed on natural food and additionally they are fed with bread cereals. In the literary review I will describe the various cereals, which are used in carp-scale reading. Further I'll try to focus on the editing posibilities of the various cereals to increase their productive effectivity. In the chapter "Results and discussion" I will describe the characteristics of growth and condition indicators of experimental fish stocks during the growing season, in experimental ponds in South Bohemia, near the city of Třeboň. Finally, I review the results and try to propose some ways to use different feeds in working practices.
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Bioimpedance methods to measure somatometric and volumetric changes
Petr, M. ; Kohlíková, E. ; Navrátil, Tomáš
Assessment of human body composition has stimulated the need for an understanding of available methodologies of estimating fat free mass and percent body fat. In our study, we found statistical significant changes of body composition evaluated by multi-frequency bioelectrical analysis in a group of athletes following creatine supplementation. These results are consistent with literature i.e. supplemented creatine effects osmolality of cell´s membranes which leads to increased intracellular water retention. Multi-frequency BIA can be considered to be an accurate and sensitive method for assessment of human body composition.
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Suplementace kreatinu u sportovce s hyperhomocysteinemií
Kohlíková, E. ; Petr, M. ; Navrátil, Tomáš ; Přistoupilová, K. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael
One month's administration of creatine (CR) in the dose of 5 g/day to 11 young active sportsmen affected their daily amount of CR and creatinine excreted into urine. The probands were divided into 4 groups, according to the amount of CR found in urine, and of folates and of vitamin B12 determined in blood. The ganges of folates and vitamin B12 were mutually reciprocal. Each group utilized CR as donor of one- and two-carbon (1C and 2C) units by means of homocysteine (HoCySH), folates, and vitamin B12, in different metabolic pathways. In 10 men the CR administration was accompanied by an increase of HoCySH level in blood, while for the last man, with accidentally discovered hyperhomocysteinemia, after the month's CR administration the HoCySH level dropped by 50 %.
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Význam lipidů v metabolismu tuků
Kohlíková, E. ; Petr, M. ; Přistoupilová, K. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael ; Navrátil, Tomáš
In course of last two years we compared values of 34 parameters, obtained from 11 young active sportsmen given 5 g of creatine (CR) daily during 1 month. Among these parameters were the products of metabolic pathways, the changes of which are connected with the development of atherosclerosis. This holds for the levels of cholesterol (CH), triacylglycerols (TAG) and homocysteine (HoCySH), and for the levels of vitamin B12 and of folates in blood.
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