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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Bioorthogonal labelling of surface receptors on living lymphocytes
 Paldusová, Kateřina ; Cebecauer, Marek (advisor) ; Benda, Aleš (referee)
The surface of cells displays high heterogeneity on chemical and geometrical levels. To understand the function of cells, we need to pay attention to the morphological features formed at the plasma membrane. To study cell surface with molecular specificity, there are plenty of imaging methods starting with the conventional wide-field microscopy through confocal microscopy, ending with super-resolution fluorescence microscopies and electron microscopies. Super-resolution microscopy studies conducted on the fixed cells provide detailed steady-state data about the cell surface nanoscopic organisation and distribution of molecules at the morphological structures. However, since cells are parts of living organisms and constantly change their properties in time and space, the information about dynamics of cellular structures and motility of molecules remains hidden when using this approach. Live-cell compatible methods are required to study dynamic changes of molecules at the single-molecule level. In this study we are focusing on the distribution and dynamics of molecules CD2 and CD4 expressed on the surface of non-stimulated T cells. The main aim of this thesis was to develop a novel method for live-cell imaging and single-molecule tracking of membrane-bound proteins in 3D and at nanoscale. With such a...
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Compartmentalisation of surface receptor signalling in T cells
 Paldusová, Kateřina ; Cebecauer, Marek (advisor) ; Janušová, Šárka (referee)
The main goal of this thesis is to introduce the topic of compartmentalisation of signalling on the surface of T lymphocytes before and during the T-lymphocyte activation by the contact with an antigen-presenting cell (APC) or a target cell. To understand the compartmentalisation of signalling, the morphology of cell surface needs to be understood first. It is believed that the surface of T cell is magnified with an abundance of membrane protrusions called microvilli. Although, little is known about their inner structure. They may have some structural features different from microvilli on the surface of APC or microvilli of intestinal brush border. These structures are further described and compared with each other along-side with some other non-microvillar membrane protrusions and extensions. The insight into three-dimensional compartmentalisation of signalling molecules in T cells is still in its early days. Some results even contradict each other. This field will require the application of advanced imaging methods in a rather comprehensive way to uncover fine organisation of these molecules before and after encountering stimulatory surface. Key words T-cell signalling, membrane receptors, cell surface morphology, microvilli, microscopy
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