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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Touha po cukru; fyziologické procesy klíštěte \kur{Ixodes ricinus} v závislosti na hostitelské glukóze
 PLAČKOVÁ, Barbora
Blood-feeding arthropods, Ixodes ricinus acquire and digest vast amounts of the host blood meal, which often exceeds their body volume up to 100 times. It seems to be an only source of energy which they need for the reproduction. I identified homologues of six sugar transporters genes encoding sugar transporters in the I. ricinus tick. Transcripts encoding sugar transporters were found in digestive tract of I. ricinus. The results of RT PCR analysis characterised tissue expression profiles of the sugar transporters while fed by blood. The sugar transporters were determined as a drug target by RNAi. In this master thesis, the use of RNAi and available sugar inhibitors did not confirm glucose importance in physiology of ticks. Membrane feeding showed that blood meal with 2-deoxy-D-glucose had a significant impact on the reproduction and viability of the ticks.
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Analýza exprese tyrosinové aminotransferázy a 4-hydroxyfenylpyruvát dioxygenázy v klíštěcích tkáních a vývojových stádiích pomocí RT-qPCR
PLAČKOVÁ, Barbora
Blood-feeding arthropods acquire and digest vast amounts of host blood meal, which often exceeds their body volume up to 100 times. Blood protein hydrolysis liberates amino acids which are recycled for de novo protein synthesis or are further catabolised. Transcripts encoding enzymes of tyrosine catabolism were found to be substantially enriched in digestive tract of blood-feeding hemipteran Rhodnius prolixus. This catabolic pathway was validated as a drug target by RNAi and viability assay with available inhibitors. In this bachelor thesis, I identified homologues of two genes encoding the first two enzymes of tyrosine catabolism, tyrosine aminotransferase and hydroxyphenylpyruvate dioxygenase, in the Ixodes ricinus tick and characterised their tissue expression patterns. Using RNAi, it was confirmed that HPPD is essential for tick survival after detachment of the host.
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