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Proteomic analysis of myocardial integral membrane proteins
Oliva, Tomáš ; Petrák, Jiří (advisor) ; Pompach, Petr (referee)
Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality in Europe. Over 4 million people die from CVDs annually and another 11 million people develops CVDs every year. These numbers show that there is a need for better diagnostic, prognostic and predictive biomarkers and, more importantly, a need for new and more efficient drugs. Integral membrane proteins (IMPs) are ideal candidates for new drug targets. However, a study of IMPs represents a major challenge in current proteomics. This challenge is associated with the low abundance of IMPs, their low solubility in aqueous solvents and the absence of trypsin cleavage sites in their transmembrane segments. To overcome these issues, methods that selectively target either N-glycosylated extra-membrane segments (CSC, SPEG, N-glyco-FASP) or transmembrane segments (hpTC) were developed. In this thesis we employed a combination of two N-glyco-capture methods (SPEG and N-glyco-FASP) performed on two different samples (membrane-enriched fraction and total tissue lysate) with analysis of membrane-embedded IMP segments by hpTC and with standard non-targeted "detergent+trypsin" approach to analyze rat myocardial membrane proteome. We also performed an evaluation of employed methods for preparation of membrane fraction by western blot...
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Proteomic analysis of myocardial integral membrane proteins
Oliva, Tomáš ; Petrák, Jiří (advisor) ; Pompach, Petr (referee)
Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality in Europe. Over 4 million people die from CVDs annually and another 11 million people develops CVDs every year. These numbers show that there is a need for better diagnostic, prognostic and predictive biomarkers and, more importantly, a need for new and more efficient drugs. Integral membrane proteins (IMPs) are ideal candidates for new drug targets. However, a study of IMPs represents a major challenge in current proteomics. This challenge is associated with the low abundance of IMPs, their low solubility in aqueous solvents and the absence of trypsin cleavage sites in their transmembrane segments. To overcome these issues, methods that selectively target either N-glycosylated extra-membrane segments (CSC, SPEG, N-glyco-FASP) or transmembrane segments (hpTC) were developed. In this thesis we employed a combination of two N-glyco-capture methods (SPEG and N-glyco-FASP) performed on two different samples (membrane-enriched fraction and total tissue lysate) with analysis of membrane-embedded IMP segments by hpTC and with standard non-targeted "detergent+trypsin" approach to analyze rat myocardial membrane proteome. We also performed an evaluation of employed methods for preparation of membrane fraction by western blot...
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Studie návrhu povodňového plánu vybraného území - Městská část Brno-sever
Oliva, Tomáš
A flood plan is one of the fundamental documents of flood protection. This thesis deals with the design of flood protection and flood protection plan, which in practice will help to correct coordination of all members involved in flood protection. The first part of the thesis is a literature review, which deals with the general characteristics of floods, their origins, what kinds of floods distinguish the conditions under which they originate and what characterizes them. There is also the issue of flood protection, Integrated Rescue System IZS and the legislation that relates to this. The next section is a brief description of the area. The territory chosen for this work is district Brno-sever. Basic documents and materials for the investigation are provided by this district and subsequently studied. Followed by field researche of flow control's whatch spots and inventory of materials for flood protection control. After reading all the materials of the flood plan of Brno-sever it was found, that some of the data are no longer valid and will need to be modified or supplement. The main problem was the issue of flood commission. Specifically, which representatives should be appointed to this committee, what is expected of them and what are their exact duties. Following these findings I prepared a clear methodology for the flood commission, in which is precisely defined, from which department this man should be and what are his tasks. The conclusion summarizes the importance of a flood protection plan. Flood protection is becoming, along with changing climatic conditions still thornier issue, and despite the fact that Brno was not offten affected by floods in the recent past, the aim should be that all inhabitatns would be prepared for this unpleasant possibility.
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