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Studies of S. cerevisiae BR-S strain with deletion of SIR2 gene
Novotná, Pavla ; Kuthan, Martin (advisor) ; Pichová, Iva (referee)
Yeasts are unicellular eukaryotic microorganisms, capable of forming of organised multicellular communities, the colonies. Many yeast strains possess a characteristic colony morphology under defined living conditions. Another feature typical for many feral and pathogenic yeast strains is the ability to switch their morphotype. This phenomenon, called the phenotypic switching, contributes to a rapid adaptation to the changing harmful environment and is often connected with changes of the stress resistance or with the changes of virulence of pathogenic yeasts. Phenotypic switching can be observed even in non-pathogenic yeast Saccharomyces cerevisiae. The strain BR-F, isolated from nature, switches under laboratory conditions from fluffy to smooth morphology of the strain BR-S. This phenotypic switch is accompanied by broad changes in the phenotype. Transcriptome analyses of the strains BR-F and BR-S have shown, among others, changes in expression of the subtelomeric genes that are under control of the histone acetylases and deacetylases. My work was aimed to the histone deacetylase Sir2p, which could influence the phenotypic switching in Saccharomyces cerevisiae. The sir2 deletion mutant of the strain BR-S, prepared in our laboratory, was used for my studies. The results show, that the strain BR-S...
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Extracellular matrix in yeast populations
Novotná, Pavla ; Kuthan, Martin (advisor) ; Dvořáček, Lukáš (referee)
The microorganisms in a natural environment are frequently found in multicellular forms, most commonly in biofilms. Biofilm is characterized as a community of cells living at the interface of two environments, embedded in the extracellular matrix. ECM is a significant component of biofilms in yeast populations. Extracellular matrix acts as a protective barrier and allows cells to survive under adverse conditions and better compete with other microorganisms. It also forms an effective barrier against antibiotics and other harmful substances, what makes biofilms a serious problem in medicine and industry. Formation of the matrix may be influenced by the morphological forms of colonies. Increased formation of ECM is commonly connected in phenotypic switching in response to changes of their living conditions. The compositeion of the ECM is a genus-and species-specific. The main component of the matrix consists of polysaccharides and proteins.
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The Economy of Churches and Religious Societies in the Context of so-called Church Restitution
Novotná, Pavla ; Deverová, Lenka (advisor) ; Dohnalová, Marie (referee)
This diploma thesis is focused on the issue of the economy of churches and religious societies in the context of so-called church restitution. The first section focuses on the position of churches and religious societies within civil society, the legislative framework of churches and religious societies, the church restitution and their current form, as well as the economy of churches and religious societies. The empirical section attempts to answer the outlined research questions. It explains the consequences of church restitution for the selected sample of churches in relation to the church economy, and it focuses on the church entrepreneurship in the context of church restitution.
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Design and experimental verification of usability testing methodology for mobile contactless NFC payments
Novotná, Pavla ; Gála, Libor (advisor) ; Zich, Martin (referee)
The purpose of this work is the design and experimental verification usability testing methodology of mobile contactless NFC payments. Given that this is the very innovative and flexible region (m-commerce and the usability of mobile devices) we need to develop a methodology to adapt the methods and usability testing standards for the specifics of mobile devices. The benefit of this work is to link the usability and m-commerce and the creation of a methodology for mobile devices usability testing. This work can be beneficial for companies that decides to enter the mobile payments market (as for instance a firm MOPET CZ rather unexpectedly made with the mobito project) and also for companies that are already dealing with m-commerce. This work can be also useful as a matrix for creating own testing methodology for mobile devices, even for other purposes.
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Krajinně-ekologické‚ vodohospodářské‚ ekonomické a legislativní hodnocení záměru výstavby kanálu Dunaj - Odra - Labe
ŠINDLAR s.r.o, Býšť ; Servus, Michal ; Zeman, Jan ; Šťovíčková, Leona ; Novotná, Pavla ; Zapletal, Jan ; Šindlar, Miloslav
Přílohy obsahující tabulky s přehledy těchto lokalit ovlivněných průplavem DOL: MZCHÚ, evropsky významné lokality, ptačí oblasti, VZCHÚ, přírodní parky, lokality s výskytem chráněných a ohrožených druhů lumbricidae, lokality s výskytem chráněných a ohrožených druhů crustacea, carabidae, odonata, lokality s výskytem významných zástupců druhů edafonu, s výskytem chráněných a ohrožených druhů amphibia, reptilia, aves, mammalia, lokalit s výskytem chráněných a ohrožených druhů rostlin. Hydrologické ovlivnění úseku toků se zástupci mollusca a se zástupci pisces.
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Air Rescue Service and its coordination with the ground based rescue personnel
NOVOTNÁ, Pavla
The Air Rescue Service is now an integral part of the Emergency Medical Service in the Czech Republic. At present the number of primary interventions in which the two components have to cooperate has been increasing. As most cases are serious, life-threatening events, it is crucial for the two components to create a collaborative unit. Therefore it is necessary to ensure an excellent level of communication and cooperation between them. This can be achieved only if both partners know, employ and respect certain rules of the partner´s cooperation. In the theoretical part the thesis deals with history and activities of the Air Rescue Service and its cooperation with the ground based component. The objective of my thesis was to survey and evaluate the level of communication and cooperation between the Air Rescue Service crews, the ground based components of the Emergency Medical Service and the Emergency Command Centre. Quantitative research was conducted through the use of questionnaires. The questionnaires were distributed to selected Emergency Medical Service and Air Rescue Service centers in the South Bohemian region and the Vysočina region. The questionnaires were addressed only to paramedics. The outcomes of the research prove that paramedics consider the communication level and the level of cooperation between the ground based components, the Air Rescue Service and the Emergency Command Centre to be adequate. It was also found that if an error occurs in communication, in the majority of cases human failure is responsible. I hope the findings of my thesis can help paramedics get an overview of this topic, or supplement their knowledge of this issue
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Possibilities of IT support in a law firm
Novotná, Pavla ; Gála, Libor (advisor) ; Šurmanová, Michaela (referee)
The aim of this work is to prepare business process analysis of companies in the field of advocacy and to assess the support of information technology, which offers software solutions available on the Czech market. Before writing this work I first studied the essential literature concerning the management of law firms, particularly regarding the possibility of process management in these companies. Then I studied the literature on process management and modeling. After a literature search, I attended a meeting with a midsize law firm manager, who provided me with valuable information about the specifics and problems in the field. My main goal was to emphasize the uniqueness of the law firms field, where the functional management model prevails over the process management model, to design general process model, to map the key processes of the functions of the software solutions available on the Czech market and to discuss the need for or the appropriateness of introducing process management in companies in advocacy field. Chapter Theoretical background and issues of the field includes introduction to the issue of functional and process company management in the law firm field, and explains how broad concept the law firm may be, and introduces the readers with the critical factors in the implementation of process management. The reader will find here a description of various types of law firms from different angles. Process analysis is a chapter devoted to identifying and supporting key processes of law firm and to the detailed analysis of key processes. The following chapter entitled Information technology as a law firm support provides an overview of several IS specializing in the law firms and lists their common functions that are appropriate to support the processes of law firm by information technology. In the last chapter entitled Possibilities and limitations of mapping IT resources of the processes outlined one of many possible solutions to support key processes of law firm by information technologies, and discussed the limits on the introduction of process management in companies providing services in the field of advocacy, which result from the variability of these companies.
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The importance and process of an Internal Audit in financial institution
Novotná, Pavla ; Burgerová, Jiřina (advisor) ; Zeman, Karel (referee)
The Theses deals with Internal Auditing, as it performed in financial institution, specifically in a bank. For the purposes of an Internal Audit process analysis Česká spořitelna has been chosen. The objective of the Theses is to assess what importance does Internal Auditing represent for Česká spořitelna and analyze in detail the individual stages of Internal Audit process. In the introductory part of the Theses, the Internal Audit is described in general. There is stated, among others, a definition of Internal Audit, the Framework for Professional Practice of Auditing and the Internal Auditor's Profile. The main part of the Theses is focused on Internal Audit process performed in Česká spořitelna. At the end of the Theses, a practical example presenting a continuous assessment of Internal Audit Quality is stated.
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