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Adenosine methylation (m6A) in plant mRNA
Holoborodko, Anastasiia ; Růžička, Kamil (advisor) ; Náprstková, Alena (referee)
In eukaryotic organisms, there are known more than 200 internal post-transcriptional modifications of RNA. N6-methyladenosine (m6 A) is the most common modification in mRNA. In plants, m6 A affects many aspects of mRNA processing, such as mRNA splicing, alternative polyadenylation, export from the nucleus, its overall stability or translation. Adding, removing, and perceiving of m6 A are handled by protein complexes containing methyltransferases (writers), demethylases (erasers), and m6A-binding proteins (readers), respectively. Dynamic regulations of m6 A have a significant effect on plant development. Also, m6 A exerts its role in response to abiotic stress and viral infection. This thesis summarizes current knowledge on m6A in plants in light of the latest advances in animal experimental models.
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ALBA-family proteins and their role in male gametophyte development
Náprstková, Alena ; Honys, David (advisor) ; Rothová, Olga (referee)
Alba family proteins are highly conserved in all domains of life. They are involved in RNA metabolism in Archae and Eucarya, while they are involved in the chromatin organisation in Crenarchaea. In animals, ALBA proteins were identified to associate with RNase P/MRP subunits. The objective of my thesis was the characterization of ALBA family proteins in a model plant Arabidopsis thaliana. The Arabidopsis genome contains six genes with close homology, three from Rpp20-like subfamily and three of Rpp25-like subfamily. Here I present the localization of GFP-fused proteins in Arabidopsis stable lines harbouring constructs cloned by Gateway® Technology. ALBA proteins were localized in the cytoplasm and undefined particles in root differentiation zone and in mature pollen. The characterization of the respective T-DNA insertion lines did not reveal significant phenotype defects in growth and development of sporophyte and gametophyte in comparison to Columbia-0 plants, probably because of likely functional redundancy od the paralogs. Expression profiles and localization of ALBA proteins suggest their possible role in differentiation and dehydration stress response in Oryza. They were also observed to associate with repressed mRNA transcripts in storage EPP particles. Collectively, I propose the likely role...
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