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Influence of plasma activated water on fungal diseases
Moskvina, Anastasia ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This bachelor thesis focuses on influence of plasma treated water on fungal diseases. The theoretical part is dedicated to plasma-liquid interactions, properties and application of plasma activated water and its influence on different microorganisms. Plasma activated water contains reactive oxygen species which cause inactivation of living cells, making it a potential sterilizer. The experimental part of this work compares the effectiveness of three plasma treated water preparation techniques. Dielectric barrier discharge system was used to activate water above its surface. For the under the surface activation, a two-electrode system of both alternating and direct current was used. Plasma treated water was then used to prepare a suspended mixture with Aspergillus niger spores. The latter was then cultivated on agar plates for 72 hours. The overall effect was evaluated in amounts of colony forming units. The experimentally obtained data was processed and discussed in the results and discussion section of the thesis. It was found that the underwater surface activation had more significant effect on mold deactivation, which corresponds with the information contained in the theoretical part. Although the effect of dielectric barrier discharge was not as promising, all three methods used led to a decrease of colony forming units in comparison to the non-treated control sample.

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