|
|
Cosmetics preparations against acne
Měšťánková, Zuzana ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
Hemp is a plant containing a number of active substances that can be used in cosmetics and pharmacy. The subject of this bachelor thesis is the multiplication of active substances by mixing cannabis with herbs that have anti-inflammatory effects. The theoretical part contains a description of the determined antioxidants and cannabinoids. The experimental part focuses on measuring the content of these substances in extracts created from the dry parts of herbs and hemp variety Ferimon. The work is also focused on the preparation of a cream containing cannabis and herbal extracts, which are aimed at inhibiting the formation of acne. The antimicrobial effect of creams and extracts was investigated on bacteria Propionibacterium acnes and Micrococcus luteus. The analysis of the extracts shows that the largest amount of active substances contained a mixture of mountain arnica and hemp, which also showed a good antimicrobial effect against both types of bacteria. The creams showed a low inhibitory effect due to the low content of active substances.
|
|
|
Preparation and characterization of packaging films based on beta-keratin
Měšťánková, Zuzana ; Mikulíková, Renata (referee) ; Kovalčík, Adriána (advisor)
This diploma thesis deals with keratin isolation from poultry feather waste and preparation of keratin-polymers composite films utilizing isolated keratin. The main goal was to isolate keratin from poultry feathers by hydrolysis and compare yield of diverse hydrolysis types. The most efficient was hydrolysis using 0.5% sodium hydroxide with yield 480 g of keratin per 1 kg of chicken feathers. Structure, morphology and thermal properties of isolated keratin was analyzed. Comparison with protein molecular weight standard it was revealed that hydrolysis cleaved keratin to macromolecules smaller than 15 kDa. Scan electron microscopy showed that size of lyophilized keratin particles was in the order tens of µm and had an irregular shape. Further, composite films based on isolated -keratin and other polymers were prepared. Thermal analysis showed, the addition of keratin significantly reduced the thermal stability of composite films. Analyses revealed that Polyvinyl alcohol composite films reached the best mechanical properties. For example, an addition of 0.25 g of keratin per 1 g of polyvinyl alcohol increased the tensile elongation by up to 68.9%. Moreover, the film based on chitosan and keratin had antibacterial properties. The results demonstrate that it is able to efficiently isolate keratin from poultry feather waste by alkaline hydrolysis and isolated keratin can be utilized to prepare potentially applicable keratin-polymers composite films.
|
|
|
Preparation and characterization of packaging films based on beta-keratin
Měšťánková, Zuzana ; Mikulíková, Renata (referee) ; Kovalčík, Adriána (advisor)
This diploma thesis deals with keratin isolation from poultry feather waste and preparation of keratin-polymers composite films utilizing isolated keratin. The main goal was to isolate keratin from poultry feathers by hydrolysis and compare yield of diverse hydrolysis types. The most efficient was hydrolysis using 0.5% sodium hydroxide with yield 480 g of keratin per 1 kg of chicken feathers. Structure, morphology and thermal properties of isolated keratin was analyzed. Comparison with protein molecular weight standard it was revealed that hydrolysis cleaved keratin to macromolecules smaller than 15 kDa. Scan electron microscopy showed that size of lyophilized keratin particles was in the order tens of µm and had an irregular shape. Further, composite films based on isolated -keratin and other polymers were prepared. Thermal analysis showed, the addition of keratin significantly reduced the thermal stability of composite films. Analyses revealed that Polyvinyl alcohol composite films reached the best mechanical properties. For example, an addition of 0.25 g of keratin per 1 g of polyvinyl alcohol increased the tensile elongation by up to 68.9%. Moreover, the film based on chitosan and keratin had antibacterial properties. The results demonstrate that it is able to efficiently isolate keratin from poultry feather waste by alkaline hydrolysis and isolated keratin can be utilized to prepare potentially applicable keratin-polymers composite films.
|
|
|
Cosmetics preparations against acne
Měšťánková, Zuzana ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
Hemp is a plant containing a number of active substances that can be used in cosmetics and pharmacy. The subject of this bachelor thesis is the multiplication of active substances by mixing cannabis with herbs that have anti-inflammatory effects. The theoretical part contains a description of the determined antioxidants and cannabinoids. The experimental part focuses on measuring the content of these substances in extracts created from the dry parts of herbs and hemp variety Ferimon. The work is also focused on the preparation of a cream containing cannabis and herbal extracts, which are aimed at inhibiting the formation of acne. The antimicrobial effect of creams and extracts was investigated on bacteria Propionibacterium acnes and Micrococcus luteus. The analysis of the extracts shows that the largest amount of active substances contained a mixture of mountain arnica and hemp, which also showed a good antimicrobial effect against both types of bacteria. The creams showed a low inhibitory effect due to the low content of active substances.
|
guest ::
