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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Development of method for determining aminoacid loading in solid phase peptide synthesis
 Mácha, Hynek ; Nesměrák, Karel (advisor) ; Křížek, Tomáš (referee)
A simple method has been developed to determine amino acid loading in solid phase peptide synthesis. The method is applicable for the most common type of synthesis, which use FMOC as protective group and piperidine as a deprotecting agent. Both products of deprotection reaction are separated by HPLC and determined using an UV detector; an internal standard is added. The method gives true values that have been verified by an independent method. The RSD is 1.52%. The method is more accurate than the published methods and allows the determination from the waste of synthesis. The employing of the internal standard eliminated the necessity of dilution accuracy or known volumes.
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The Comparison of Methods for Determination of Diclofenac in Pharmaceutical Samples
 Mácha, Hynek ; Nesměrák, Karel (advisor) ; Rychlovský, Petr (referee)
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