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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Optimization of a method for iodine determination in urine in a flow system using solid phase extraction
 Lipenská, Zuzana ; Horstkotte Šrámková, Ivana (advisor) ; Chocholouš, Petr (referee)
Department: Department of Analytical Chemistry Candidate: Bc. Zuzana Lipenská Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Consultant Doc. PharmDr. Hana Sklenářová, Ph.D. Title of diploma thesis: Optimization of a method for iodine determination in urine in a flow system using solid phase extraction Iodine is an important element for the human body to ensure proper functioning of thyroid gland. This organ produces the hormones thyroxine and triiodothyronine necessary for proper brain development and correct function of other hormones (catecholamines). The human body is unable to produce iodine, so the only income is via food. Incorrect level of this element can cause serious health complications, such as congenital mental retardation. Body level of iodine is most commonly determined from urine, which requires a reliable and sensitive method. The most widely used method is spectrophotometric analysis based on the Sandell-Kolthoff reaction. For urinary iodine determination, it is advantageous to use flow methods to ensure sufficient measurement sensitivity and automation. Most methods of iodine monitoring use toxic reagents or generate toxic waste. The aim of this diploma thesis was to optimize the hydrodynamic and chemical parameters of a method for urine iodine analysis using...
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Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases
 Lipenská, Zuzana ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Zuzana Lipenská Title: Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases Bachelor's Thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The aim of the study of HLA B27 antigen in patients investigated for suspected rheumatological disease in 2015. Methods: Patient samples examined for the presence of antigen HLA-B27 in the HLA laboratory of the Transfusion Department, University Hospital Hradec Kralove are processed using the serological method. First is used the isolation of peripheral blood lymphocytes using a density gradient, thereafter lymphotoxical test with peripheral lymphocytes. Results: In 2015 were examined 1,329 patients. 207 persons had the HLA-B27, 1122 persons did not. Of the total number of examined patients were examined 754 women and 575 men. Positive was found 108 women and 99 men. The highest incidence of HLA-B27 was observed in the group of 20-29 years old, another slight increase was in the group of 10-19 years old and 40-49 years old. The examined persons had mainly a group diagnosis of disease of the musculoskeletal system with the most frequently diagnosed dorsalgia (M54). Conclusions: The association between HLA-B27 antigen and the deseases has...
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