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Study of the prophylactic effect of glycoclusters on a model of bacterial adherence
Kurucová, Michaela ; Hodek, Petr (advisor) ; Kubíčková, Božena (referee)
Cystic fibrosis (CF), an autosomal recessive genetic disorder, arises from mutations in the CFTR gene encoding the CFTR protein, which primarily functions as a chloride channel in the body. The malfunction of chloride ion transport leads to multiple organ dysfunctions, with the most significant impact on the respiratory system. A hallmark of CF is the increased adhesion of bacteria, especially Pseudomonas aeruginosa (PA), to the lung epithelium, resulting from the formation of dense mucus and glycosylation modification in the lungs. The pathogenicity of PA is significantly contributed to by its virulent factors, specifically PA-IL and PA-IIL lectins, which facilitate adhesion to host cells by binding to surface receptors containing D-galactose (PA-IL) or L-fucose (PA-IIL). The main objective of this study was to demonstrate the ability of synthetically prepared multivalent inhibitors based on fucose and galactose to inhibit PA adhesion to lung epithelial cells. Trivalent glycoclusters designed to target the PA lectins were studied ex vivo using an adhesion test employing immortalized epithelial cells - the CuFi-1 (from a CF patient) and NuLi-1 (from a healthy individual) cell lines. A control PA strain, denoted as PAK (ST 1763), was utilized to monitor bacterial adhesion. For visualization and...
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