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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Mechanisms of exodermal response to nutrient availability in the environment
 Gargoš, Ondřej ; Tylová, Edita (advisor) ; Kummerová, Marie (referee)
in English The apoplastic barriers of the root (endodermis and exodermis) represent an important regulatory mechanism for the uptake of water and nutrients from the environment, ensuring its selectivity. In addition, both layers respond to stress factors by altering its rate and degree of cell wall modification, which affects the transport properties of the root and represents adaptive plants to high heterogeneity of the soil environment. Apoplastic barriers also respond to the availability of mineral nutrients. This issue has recently been intensively studied and a number of ambiguities persist. Interestingly, the deficiency of some mineral nutrients stimulates the differentiation of barriers, while the deficiency of other mineral nutrients delays the differentiation. In addition, different plant species react differently to the deficiency of the same element. Another interesting aspect is the fact that the reaction of the endodermis and exodermis is localized and takes place mainly in that part of the root system which is directly exposed to the stress factor. This phenomenon has been observed with cadmium toxicity, but more recently with local nutrient deficiencies (nitrogen and potassium) in Zea mays. This diploma thesis deals with the functional significance of localized enhancing or delaying...
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Characterization of programmed cell death during lyzigenous aerenchyma formation
 Lenochová, Zuzana ; Votrubová, Olga (advisor) ; Čiamporová, Milada (referee) ; Kummerová, Marie (referee)
Abstract____________________________________________________________________ 2 ABSTRACT: Zea mays is generally considered to be a plant with inducible lysigenous aerenchyma formation. The degradation of some cortical cells is triggered by environmental conditions, usually in the form of stress (submergence etc.). These cells die in a process that shows signs characteristic for programmed cell death, such as nuclear DNA fragmentation or apoptotic ultrastructural alterations. Aerenchyma formed in primary roots of thirteen examined maize accessions, irrespective of cultivation conditions. The aerenchyma fraction correlated with the root length, not with its age. The dependence of aerenchyma formation on the presence of this phytohormone was proved by using an inhibitor of ethylene synthesis (AOA). It was found out that the aerenchyma formation depended on light conditions and that the lysigenous intercellulars intercellular spaces colocalized with areas with cells with characteristically fragmented nuclear DNA (TUNEL-positive nuclei). In experiments using the TUNEL reaction it was necessary to determine new dilution of the enzymatic mixture for the examined plant material. Only the observation of surface planes of free-hand root sections was considered relevant in both TUNEL-TMR and TUNEL-AP assays. TUNEL-AP...
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