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Tau protein and its variants in the diagnosis of Alzheimer's disease
Krestová, Michala ; Říčný, Jan (advisor) ; Fialová, Lenka (referee) ; Balaštík, Martin (referee)
It is accepted that fibrillar aggregated tau is the best histopathological correlate of the onset and progression of dementia. Tau protein was long regarded as an intracellular protein with several functions inside of cells. New evidence suggests tau secretion into the extracellular space. It is plausible that both intracellular and extracellular forms of tau protein contribute to AD neurodegeneration. The truncated/fragmented forms of tau protein are prone to self- aggregate and form soluble oligomers which are now considered the toxic agents that spread the pathology in AD and other tauopathies. In addition, immunologic abnormalities including defective immune regulation and autoimmunity have been demonstrated in AD patients. Therefore, we have studied the role of various extracellular forms of tau protein and antibodies against them in AD. Firstly, we showed that antibodies isolated from intravenous IgG (IVIG, product Flebogamma) and plasma of older cognitively healthy persons (controls) were reactive with pathological soluble aggregates (oligomers) of tau protein present in the brain of AD patients. On the contrary, isolated antibodies from the plasma of AD patients revealed reactivity with lower molecular weight (LMW, monomeric) tau forms found in brain tissue. Moreover, the antibodies from...
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Tau protein and its variants in the diagnosis of Alzheimer's disease
Krestová, Michala ; Říčný, Jan (advisor) ; Fialová, Lenka (referee) ; Balaštík, Martin (referee)
It is accepted that fibrillar aggregated tau is the best histopathological correlate of the onset and progression of dementia. Tau protein was long regarded as an intracellular protein with several functions inside of cells. New evidence suggests tau secretion into the extracellular space. It is plausible that both intracellular and extracellular forms of tau protein contribute to AD neurodegeneration. The truncated/fragmented forms of tau protein are prone to self- aggregate and form soluble oligomers which are now considered the toxic agents that spread the pathology in AD and other tauopathies. In addition, immunologic abnormalities including defective immune regulation and autoimmunity have been demonstrated in AD patients. Therefore, we have studied the role of various extracellular forms of tau protein and antibodies against them in AD. Firstly, we showed that antibodies isolated from intravenous IgG (IVIG, product Flebogamma) and plasma of older cognitively healthy persons (controls) were reactive with pathological soluble aggregates (oligomers) of tau protein present in the brain of AD patients. On the contrary, isolated antibodies from the plasma of AD patients revealed reactivity with lower molecular weight (LMW, monomeric) tau forms found in brain tissue. Moreover, the antibodies from...
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