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Epidemiological and Clinical Aspects of Food Allergy
Bělohlávková, Simona ; Panzner, Petr (advisor) ; Krčmová, Irena (referee) ; Rybníček, Ondřej (referee)
The prevalence of food allergy is rising rapidly in last two decades. It almost doubled between 1999 and 2011. It currently occurs in 6-8 % of children and 3-6 % of adults in Europe, and about 10 % in the US. Food allergy is more common in children than in adults and it's prevalence differs in industrialized/westernized countries. The spectrum of most frequent triggering foods, symptoms of food allergy and types of food sensitization are geographically different. Until now, we have only limited data available of food allergies in patients in the Czech republic. The aim of the study was to gather epidemiological data of food allergies in patients from the Czech republic. We used electronic registry DAFALL (Database of Food Allergies) to prospectively collect data from patients with newly diagnosed food allergy. During the 3 years of follow up, 1747 patients with IgE or non-IgE mediated food allergy were enrolled into the registry. 22,1 % of them were infants, 26,1 % children at the age of 1 to 6 years, 24,9 % kids between 7 and 18 years and 26,9 % adults. We compared the most frequent types of food triggering reactions in different age groups, severity of symptoms, the prevalence of IgE sensitization considering available data from the other parts of Europe. We have found some specific patterns of...
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Hereditary angioedema - diagnostics, therapy and life quality of patients with this diagnosis
Pilát, Martin ; Krčmová, Irena (advisor) ; Králíčková, Pavlína (referee)
Charles University Faculty of Pharmacy in Hradec Králové Departmen of Biological and Medical Sciences Student: Martin Pilát Supervisor: Irena Krcmova, M.D. Title: Hereditary angioedema - diagnostics, therapy and life quality of patients with this diagnosis The theoretical part of this diploma thesis presents basic information about Hereditary Angioedema (HAE). It includes a short history, genetics, pathophysiology, different types of HAE, the clinical picture, description of laboratory methods used to diagnose HAE and current therapy options. The practical part consists of a description of the origin and development of methods for evaluation of patients with the Angioedema and statistical evaluation of a questionnaire filled by 66 patients with Hereditary Angioedema at four outpatient departments of faculty hospitals focused on this disease. The main aim of the thesis is to learn more about the quality of life of patients with HAE today. The discussion then works with the questionnaire results and compares them with data from other international sources. The processed results lead to the conclusion that the areas of daily life which are impaired the most are physical activities and broken sleep at night with the following tiredness during the day. Just as important is the negative influence on...
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Bazotests and lymphocyte transformation tests linked to drug intolerance
Honsárková, Tereza ; Krčmová, Irena (advisor) ; Andrýs, Ctirad (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Science Candidate: Bc. Tereza Honsárková Supervisor: prim. MUDr. Inrena Krčmová, CSc. Title of diploma thesis: Bazotests and lymphocyte transformation tests linked to drug intolerance Basophil activation tests and lymphocyte transformation tests are methods that allow drug allergy diagnosis. The right diagnosis is crucial for treatment. Drug allergy is often associated with β-lactam antibiotics hypersensitivity and especially with penicillins. The aim of this diploma thesis is to provide actual statistics based on selected patient medical reports. Those patients were completely clinically and laboratory examined. The main condition for data selection was the execution of skin tests for suspicion of drug allergy caused by penicillin-like antibiotics at the Institute of Clinical Immunology and Allergology of the Faculty Hospital Hradec Králové in 2017. Patients' anamnesis, skin tests and laboratory examination focused on basophil activation tests and lymphocyte transformation was composed. Statistics of 25 patients shows beneficial informations. Delayed manifestations are the most prevalent (63 %) in patients which is interesting. However the patients with positive skin test on major determinant had acute...
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Systemic and Local Allergic Insect Sting Reaction From The Epidemiological, Diagnostic a Therapeutic Point of View
Vachová, Martina ; Panzner, Petr (advisor) ; Kučera, Petr (referee) ; Krčmová, Irena (referee)
Hymenoptera venom allergy threatens the patients with severe, often life threating anaphylactic sting reaction. Proper diagnostics and treatment are therefore crucial for the patient with this diagnosis and should be performed by an experienced allergist. We aimed to provide a comprehensive look at these patients, from the epidemiological, diagnostic and therapeutic point of view. Initially, we focused on predictors of severe systemic sting reactions. Then we followed by evaluation of the utility of available laboratory diagnostic methods and by monitoring of immunological parameters during venom immunotherapy. We met all these goals. We detected higher age, sting localization in the head region and male gender as predictors of severe systemic sting reaction. We also evaluated the clinical utility of all diagnostics methods, and we even suggested the possible way, how to optimize the diagnostic procedure in patients with Hymenoptera venom allergy. We noticed significant changes in immunological parameters during venom immunotherapy. Due to a low number of stings during treatment, we have not been able to evaluate the clinical relevance of these changes yet.
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Bazotests and lymphocyte transformation tests linked to drug intolerance
Honsárková, Tereza ; Krčmová, Irena (advisor) ; Andrýs, Ctirad (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Science Candidate: Bc. Tereza Honsárková Supervisor: prim. MUDr. Inrena Krčmová, CSc. Title of diploma thesis: Bazotests and lymphocyte transformation tests linked to drug intolerance Basophil activation tests and lymphocyte transformation tests are methods that allow drug allergy diagnosis. The right diagnosis is crucial for treatment. Drug allergy is often associated with β-lactam antibiotics hypersensitivity and especially with penicillins. The aim of this diploma thesis is to provide actual statistics based on selected patient medical reports. Those patients were completely clinically and laboratory examined. The main condition for data selection was the execution of skin tests for suspicion of drug allergy caused by penicillin-like antibiotics at the Institute of Clinical Immunology and Allergology of the Faculty Hospital Hradec Králové in 2017. Patients' anamnesis, skin tests and laboratory examination focused on basophil activation tests and lymphocyte transformation was composed. Statistics of 25 patients shows beneficial informations. Delayed manifestations are the most prevalent (63 %) in patients which is interesting. However the patients with positive skin test on major determinant had acute...
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The effect of the diseases in infancy on the prevalence of allergies in children
Kotrchová, Veronika ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
Author: Veronika Kotrchová Title: The effect of the diseases in infancy on the prevelance of allergies in children Diploma work Charles University in Prague, Faculty of Pharmacy in Hradec Králové, study program Pharmacy Background: To find out if diseases in infancy are protective or risk factors for prevelance of allergy. We interested mainly in infectious diseases. We compared our results with so called Hygiene hypothesis, which claims that contact with microbes in early childhood boosts immature immune system due to activation of Treg lymphocytes. Methods: We sent 2793 questionnaires to 69 elementary schools all over the Czech republic. 1697 of them returned to us. We defined two groups of respondents allergics and non-allergics. For categorization we used specific criterions. According to these criterions 420 respondents were named as allergic and 774 respondents as non-alergic. For better identification if concrete disease played role as protective or risk factor for development of allergy, we chose another specific criterions. Results: We didn't prove any statistical significance between diseases in infancy and the later prevelance of allergies except for bronchitis. According to our results bronchitis in first year of life played role as risk factor for development of allergic symptoms after...
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The effect of the diet on the prevalence of allergies in children
Machová, Alexandra ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Alexandra Machová Supervisor: PharmDr. Petr Jílek, CSc. Title of diploma thesis: The effect of the diet on the prevalence of allergies in children BACKGROUND: The aim of our work was to investigate a connection between child's nutrition and development of allergy and to confront our results with recent findings in this field of science. METHODS: We distributed 2793 self-made questionnaires to 69 schools all around the Czech Republic. The target group were children in 7th and 8th grade and their parents who responded retrospectively on a wide variety of topics. We got back 1697 questionnaires RESULTS: Consumption of this food had a negative effect on a development of allergy: yoghurts 2 - 5 times per week while breastfeeding (p < 0,05), fish less than once a week while breastfeeding (p < 0,05), meat and smoked meat 1 - 3 times a week between 4 and 6 years of age (p < 0,05). On the opposite, these had a positive (preventive) effect: no yoghurts while breastfeeding (p < 0,05), homegrown fruits while breastfeeding (p < 0,05), smoked meat occasionally at age of 2 and doing so continuously in the following years (p < 0,005). CONCLUSIONS: From a wide range of data we got only few...
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Immunological evaluation of patients in "waiting list" for kidney transplantation.
Kica, Ondřej ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
The transplantation might be in some cases the last chance to save life of the patient. This therapeutical approach is used in those organs, which are not replaceable by any alternative way. The main problem, which was not solved yet, is a rejection reaction induced in the body of the recipient, where the diferences between HLA antigens of the donor and recipient play a key role. Precise laboratory examination of the patient on waiting list before the transplantation is one of the essential factors affecting the outcome of the transplanted graft and its recipient.
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Current diagnostic possibilities of allergies.
Babjáková, Ladislava ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
UNIVERZITA KARLOVA V PRAZE FARMACEUTICKÁ FAKULTA V HRADCI KRÁLOVÉ Katedra biologických a lékařských věd MOŢNOSTI SOUČASNÉ DIAGNOSTIKY ALERGIÍ (REŠERŠE) Bakalářská práce Vedoucí bakalářské práce: PharmDr. Petr Jílek, CSc. Plzeň, 2011 Ladislava Babjáková Prohlašuji, že tato práce je mým původním autorským dílem. Veškerá literatura a další zdroje, z nichž jsem při zpracování čerpala, jsou uvedeny v seznamu použité literatury a v práci řádně citovány. Práce nebyla využita k získání jiného nebo stejného titulu. V Plzni, dne ....................... podpis................................... Děkuji PharmDr. Petru Jílkovi, CSc. a MUDr. Václavě Gutové za odborné vedení, poskytnuté informace a rady. ABSTRAKT Univerzita Karlova v Praze Farmaceutická fakulta v Hradci Králové Katedra biologických a lékařských věd Kandidát: Ladislava Babjáková Školitel: MUDr. Václava Gutová Název bakalářské práce: Možnosti současné diagnostiky alergií Cílem této práce bylo vytvořit přehled možností diagnostiky alergií, která je v současné době prováděna v ambulantních ordinacích na odděleních alergologie a imunologie. Vytvořit přehled metod, které jsou standardně používané při laboratorní diagnostice alergií a dále metod užívaných pro výzkumné účely. V této práci jsou použity především informace, které byly publikovány v posledních letech...
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Autovaccines
Bureš, Martin ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medicine Sciences Candidate: Martin Bureš Supervisor: PharmDr.Petr Jílek,CSc., MUDr. Eva Budayová Title of diploma thesis: Autovaccine In 2012, 108 years have passed since the first use autovaccines. This therapy is mainly used to treat chronic allergic response to antigenic substances batteries. The methodology that is used to prepare: Příprava mikrobiálního antigenního komplexu k diagnostice a léčbě infekčně alergických onemocnění (Maršálek, 1971) a Návrh na štandartné metódy prípravy bakteriových imunomudulačných "stock" vakcín team of authors, 1991) The theoretical part is described the production process of autovaccines in detail, skin test and administration of autovaccines. The content of this work is also a statistical evaluation of a set of samples sent to the department vaccines Institute of Clinical Immunology and Allergology University Hospital in during the years 2009, 2010 and 2011. This is detected the distribution of patients by age, gender, forms of administration, and given the diagnosis of pathogenic strains of bacteria.
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