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Role of modified autophagosomal function in patophysiology of Huntington's disease.
Kotrčová, Eva ; Motlík, Jan (advisor) ; Sládková, Jana (referee)
Huntington's disease, an autosomal dominant neurodegenerative disease, affects the cell in several toxical ways. One of them is accumulation of protein aggregates in cytoplasma, which could become a serious problem especially for long-lived cells such as neurons. Autophagy (macroautophagy) is an important catabolic pathway, crucial for cell survival. If fully functional, it should eliminate protein aggregates and reduce the toxic effect on the cell. However, recent works show that this pathway might be defective, most probably in the cytoplasmic cargo recognition. In my work I used a transgenic miniature pig model of Huntington's disease to verify the hypothesis of autophagical dysfunction in individuals suffering from Huntington's disease. I studied levels of autophagosomal markers - LC3 and p62 in mesenchymal stem cells after different autophagy stimulation treatments, and ammonium chloride was found the most effective. In addition I evaluated the effect of age of the animals on autophagic function, but no significant changes were identified, even if animal genotype was considered. Moreover I had an opportunity to study proteins levels in three porcine brain tissues - cortex, cerebellum and striatum. Even though there is no significant diference, we can observe a trend of LC3 II and p62 increase in...
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Polymorphism of STR loci on the X chromosome and its usage in forensic genetics.
Kotrčová, Eva ; Čapek, Pavel (advisor) ; Zachová, Markéta (referee)
Personal or kinship identification by STR (short tandem repeat) loci is currently a routinely used method, not only in forensic practise. Autosomal STRs are usually analyzed, Y chromosome also being popular because of his great importance (especially in genealogy) due to his recombination incapability. The X chromosome has been used least frequently in these respect, even though in specific cases, it might be more descriptive than autosomal loci. The purpose of this work is to provide a basic overview of the STR sequences and methods that are commonly used to analyze microsatellites, with emphasis on their specific behavior (if located on the X chromosome) and the subsequent possibilities of using these specifities especially in forensic genetics.
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Zvláštnosti podnikatelského prostředí, obchodní a kulturní zvyklosti: Japonsko
Kotrčová, Eva ; Mertlíková, Františka (advisor) ; Hájková, Eliška (referee)
Práce analyzuje podnikatelské prostředí Japonska a posuzuje jeho výhody a nevýhody z pohledu zahraničního podnikatele,který chce vstoupit na japonský trh.Zabývá se všeobecnými informacemi o této zemi a popisuje vývoj japonského hospodářství a jeho současný stav. Dále se věnuje zahraničnímu obchodu Japonska a jeho struktuře a perspektivním odvětvím pro zahraniční firmy. Zabývá se také obchodními vztahy Japonska a EU a ČR a podmínkami vstupu na trh. Jako poslední se práce zaměřuje na japonskou obchodní etiketu.
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