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Use of capillary electrophoresis with UV photometric and electrospray ionization mass spectrometric detection for the study of interaction of compounds
Konášová, Renáta ; Jaklová Dytrtová, Jana (advisor) ; Mikšík, Ivan (referee) ; Tůma, Petr (referee)
Capillary electrophoresis (CE) is highly efficient separation method based on the different migration velocity of ions in liquid media in electric field. It is commonly used in analytical laboratories and due to the different separation principle it is applied as a complementary method to the chromatographic methods (HPLC and UHPLC). Beside the applicability of CE for quantitative/qualitative analysis, the method can be used also for physico-chemical characterization of compounds (e.g. determination of acid dissociation constants of weak electrolytes or stability constants of complexes). This work is focused on the applicability of CE methods for determination of physico- chemical characteristic of compounds (acid dissociation constants of triazole fungicides and stability constants of dibenzo-18-crown-6, benzo-18-crown-6 and 18-crown-6 ether complexes with metal ions in hydro-organic solvent mixtures) and on the possibility to use affinity CE (ACE) with electrospray ionization-mass spectrometric detection (ACE- ESI/MS) for the study of non-covalent interactions of compounds. For the online hyphenation of CE and ESI/MS, two highly sensitive CE-ESI/MS interfaces were tested: i) porous tip and ii) nano-sheath liquid flow. The ability of the CE-ESI/MS interfaces to effectively decouple spray and...
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Determination of Bitrex in conifer needles using capillary electrophoresis
Jaklová Dytrtová, Jana ; Jakl, M. ; Konášová, Renáta ; Kašička, Václav
Two extraction procedures have been developed for the sample preparation regarding the determination of Bitrex (Denatonium benzoate) on the surface of conifer needles (1) and in the biomass of the chopped conifer needles (2). The content of the Bitrex in the extracts was determined by capillary electrophoresis with UV-absorption photometric detection. The recovery of Bitrex from the needle surface is 90%, however the procedure designed for determination of Bitrex from needles is not sufficient, because the recovery is only 30 %.
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Use of capillary electrophoresis with UV photometric and electrospray ionization mass spectrometric detection for the study of interaction of compounds
Konášová, Renáta ; Jaklová Dytrtová, Jana (advisor) ; Mikšík, Ivan (referee) ; Tůma, Petr (referee)
Capillary electrophoresis (CE) is highly efficient separation method based on the different migration velocity of ions in liquid media in electric field. It is commonly used in analytical laboratories and due to the different separation principle it is applied as a complementary method to the chromatographic methods (HPLC and UHPLC). Beside the applicability of CE for quantitative/qualitative analysis, the method can be used also for physico-chemical characterization of compounds (e.g. determination of acid dissociation constants of weak electrolytes or stability constants of complexes). This work is focused on the applicability of CE methods for determination of physico- chemical characteristic of compounds (acid dissociation constants of triazole fungicides and stability constants of dibenzo-18-crown-6, benzo-18-crown-6 and 18-crown-6 ether complexes with metal ions in hydro-organic solvent mixtures) and on the possibility to use affinity CE (ACE) with electrospray ionization-mass spectrometric detection (ACE- ESI/MS) for the study of non-covalent interactions of compounds. For the online hyphenation of CE and ESI/MS, two highly sensitive CE-ESI/MS interfaces were tested: i) porous tip and ii) nano-sheath liquid flow. The ability of the CE-ESI/MS interfaces to effectively decouple spray and...
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