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Quality control of molecular biology methods in transplantation medicine
Kotrbatý, Jiří ; Kolesár, Libor (advisor) ; Schierová, Michaela (referee)
This work is focused on the description of quality control of molecular-biological methods used in transplant medicine, ie methods of HLA typing. Quality control is part of the quality management system. It consists of several components: validation, verification, internal quality control and external quality assessment. Each medical laboratory, including laboratories engaged in HLA typing, these elements must be applied to its routine operation. Validation is done before the introduction of methods and verifies whether the method is correct for the intended use. Verification is to verify that the validated method is used correctly in laboratory conditions. Internal quality control is based on an analysis of known positive and negative samples and evaluate the accuracy and precision of the whole analytical process. The external evaluation of the quality of the different laboratories analyze the same sample and the results are compared to each other. Key words: HLA typing, validation, verification, internal quality control, external quality assessment
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Clinical significance of cytokine gene polymorphism
Kolesár, Libor
Univerzita Karlova v Praze Přírodovědecká fakulta Studijní program: Doktorský studijní program v biomedicíně Studijní obor: Imunologie Mgr. Libor Kolesár Klinický význam polymorfismu cytokinových genů Clinical significance of cytokine gene polymorphism Disertační práce Vedoucí závěrečné práce/Školitel: Prof. MUDr. Ilja Stříž, CSc Praha 2012 Abstract The human genome is full of different sequence variants. They are different mainly in size but also in their influence on phenotype. The smallest unit of genetic polymorphism is single nucleotide polymorphism (SNP). SNPs represent a single nucleotide change between two alleles and might affect the gene expression. We have studied SNPs in three distinct fields as: (1) marker of risky patients after the organ transplantation, (2) diagnostic marker of patients with interstitial lung diseases (ILD) or (3) with uterine fibroid (UF). We have come to the following results. Ethnicity or even nationality plays a role in the distribution of genetic polymorphism. This must be absolutely taken into account when one would like to transfer findings of a clinical study from a certain nation or ethnic and applied them to his studied group for the comparative purposes. Our first clinical gene-association study has found that even gene polymorphism of the IL-18 gene may...
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Clinical significance of cytokine gene polymorphism
Kolesár, Libor
Univerzita Karlova v Praze Přírodovědecká fakulta Studijní program: Doktorský studijní program v biomedicíně Studijní obor: Imunologie Mgr. Libor Kolesár Klinický význam polymorfismu cytokinových genů Clinical significance of cytokine gene polymorphism Disertační práce Vedoucí závěrečné práce/Školitel: Prof. MUDr. Ilja Stříž, CSc Praha 2012 Abstract The human genome is full of different sequence variants. They are different mainly in size but also in their influence on phenotype. The smallest unit of genetic polymorphism is single nucleotide polymorphism (SNP). SNPs represent a single nucleotide change between two alleles and might affect the gene expression. We have studied SNPs in three distinct fields as: (1) marker of risky patients after the organ transplantation, (2) diagnostic marker of patients with interstitial lung diseases (ILD) or (3) with uterine fibroid (UF). We have come to the following results. Ethnicity or even nationality plays a role in the distribution of genetic polymorphism. This must be absolutely taken into account when one would like to transfer findings of a clinical study from a certain nation or ethnic and applied them to his studied group for the comparative purposes. Our first clinical gene-association study has found that even gene polymorphism of the IL-18 gene may...
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Clinical significance of cytokine gene polymorphism
Kolesár, Libor ; Stříž, Ilja (advisor) ; Holáň, Vladimír (referee) ; Mrázek, František (referee)
Univerzita Karlova v Praze Přírodovědecká fakulta Studijní program: Doktorský studijní program v biomedicíně Studijní obor: Imunologie Mgr. Libor Kolesár Klinický význam polymorfismu cytokinových genů Clinical significance of cytokine gene polymorphism Disertační práce Vedoucí závěrečné práce/Školitel: Prof. MUDr. Ilja Stříž, CSc Praha 2012 Abstract The human genome is full of different sequence variants. They are different mainly in size but also in their influence on phenotype. The smallest unit of genetic polymorphism is single nucleotide polymorphism (SNP). SNPs represent a single nucleotide change between two alleles and might affect the gene expression. We have studied SNPs in three distinct fields as: (1) marker of risky patients after the organ transplantation, (2) diagnostic marker of patients with interstitial lung diseases (ILD) or (3) with uterine fibroid (UF). We have come to the following results. Ethnicity or even nationality plays a role in the distribution of genetic polymorphism. This must be absolutely taken into account when one would like to transfer findings of a clinical study from a certain nation or ethnic and applied them to his studied group for the comparative purposes. Our first clinical gene-association study has found that even gene polymorphism of the IL-18 gene may...
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Quality control of molecular biology methods in transplantation medicine
Kotrbatý, Jiří ; Kolesár, Libor (advisor) ; Schierová, Michaela (referee)
This work is focused on the description of quality control of molecular-biological methods used in transplant medicine, ie methods of HLA typing. Quality control is part of the quality management system. It consists of several components: validation, verification, internal quality control and external quality assessment. Each medical laboratory, including laboratories engaged in HLA typing, these elements must be applied to its routine operation. Validation is done before the introduction of methods and verifies whether the method is correct for the intended use. Verification is to verify that the validated method is used correctly in laboratory conditions. Internal quality control is based on an analysis of known positive and negative samples and evaluate the accuracy and precision of the whole analytical process. The external evaluation of the quality of the different laboratories analyze the same sample and the results are compared to each other. Key words: HLA typing, validation, verification, internal quality control, external quality assessment
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