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Unqualified Supervisors, Therapists and Mediators in the Context of Social Work
KOHOUTOVÁ, Nela
This bachelor thesis deals with unqualified supervisors, therapists and mediators in the context of social work. The aim of this thesis was to find out whether and what professional public´s experience of professional such as supervisors, therapists and medators is. Furthermore, find out if they have had any experience and whether with a trained or unqualified professionals in the field. In my thesis, I set the main research question, which was what is the awareness of the professional public about unqualified supervisors, therapists and mediators in the context of social work. In the research part, I used a qualitative research stratégy and the semi-strustured interview technique. The research was carried out in Trhové Sviny a Český Krumlov. Interviews were cunducted with eight informants, trasncribed into Microsoft Word and then devided into five clusters. The research revealed that the public has experience with supervisors, therapists and mediators, but is unaware of these professionals working without the necessary qualifications. The research further revealed that qualifications are imporatnt to the professional public and they would not use the services of a professional who did not demonstrate their qualifications. It was also found that, according to the professional public, insufficient qualifications can affect the outcome of a contract, but sufficient qualifications can also affect the outcome. This thesis can be used to inform the work of supervisors, therapists and mediators. Furthermore this thesis could be used as educational materiál. Last but not least, this thesis could be used to extend the research to other regions.
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Determination of amino acids on copper-based electrodes
Kohoutová, Nela ; Dejmková, Hana (advisor) ; Schwarzová, Karolina (referee)
In this Thesis, electrochemical behavior of the flow detector made of the thin copper wires was studied. This detector is suitable for the determination of amino acids without preliminary derivatization; in this case, tyrosine was used as the model compound. Suitable activation procedure for the copper electrode was found, which assured the stable baseline with lowest possible background current. It was also found that the optimum detection potential for tyrosine determination is 0.3 V. It was shown that the mass of the copper electrode does not influence the response of the electrode. Nevertheless, according to the comparison of the theoretical and found charge, the electrochemical reaction is not quantitative. Therefore, the electrode works in amperometric and not in coulometric mode. Finally, linearity of the current response in dependence on the tyrosine concentration was confirmed in the concentration range from 1.0·10-3 mol·dm-3 to 2.0·10-5 mol·dm-3 . Limit of detection was 1.5·10-5 mol·dm-3 and limit of quantification was 5.1 ·10-5 mol·dm-3 .
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Voltammetric behaviour and determination of indole-3-butyric and indole-3-acetic acid
Kohoutová, Nela ; Zima, Jiří (advisor) ; Vyskočil, Vlastimil (referee)
This work has been based on a study of voltametric behaviour and determination of indole-3-butyric and indole-3-acetic acid with aim to use deconvolution for determination of both substances simultaneously. First measurements were made using a carbon paste electrode in Britton-Robinson buffer in the pH of 2, 4, 6, 8, 10 and 12. However, CPE is not suitable for determination of such substances because of generation of side peaks, which were possibly the result of oxidation of products of these studied substances. The carbon paste electrode was therefore replaced by a glassy carbon electrode. Considering the aim to determine both substances simultaneously, the most effective electrolyte appeared to be a 0,05M solution of sulphuric acid (pH=1,2), because there was higher peak difference of both substances in this solution than in the BR buffer (pH=2). The linearity of current response on the concentration of studied substances was confirmed by measuring a series of calibration solutions (10 - 100 μmol·dm-3 ). Found limits of detection were in micromolar levels for both substances. With the use of deconvolution, both substances could indeed be simultaneously determined. However, achieved results were highly inaccurate, not only in prepared solutions, but also in real sample.
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