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Automation of liberation tests for releasing biologically active substances from nanofibers
Kodedová, Marie ; Sklenářová, Hana (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Marie Schneidrová Supervisor: doc. PharmDr. Hana Sklenářová, Ph.D. Title of the diploma thesis: Automation of liberation tests for releasing biologically active substance from nanofibers The main goal of this diploma thesis is an automation of TTC liberation from nanofibers, using non-separation flow method sequential injection analysis. UV-VIS spectrophotometer was used for the detection of signal. Measurements were carried out using 1-3 connected Franz cells in parallel, palced in the water bath. Buffer solutions with pH 7.4 and 4.5 were used as liberation media. The liberation of TTC was realized from two different nanofiber carriers - types "A" and "AK", Difference was in their preparation process. Because of this, each of them revealed various characteristics mainly related to the amount of bound TTC and the way of its binding to the nanofiber carrier. Production of these nanofibers was realized by Technical University Liberec using the technology NanoSpiderTM . Measurements were performed under various predetermined conditions, representing the state of the human skin - two different pH (7.4; 4.5) and temperature (32řC; 37řC) values. Four different types of membrane filters were also used,...
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A study of sedimentation and flocculation of selected yeast strains by methods of complex turbidimetric analysis
Kodedová, Marie
rigorozni prace Ndzevprdce: Studium sedimentace a flokulace vybranych kmenu kvasnic metodami komplexni turbidimetricke analyzy Aulor: Mgr. Marie Kodedova Vedouciprace: Doc. Ing. Petr Sladky, CSc,, UK- MFF Komulianti: Doc. KNDr. Blanka Janderova, CSc., UK- PfF Prof. RNDr. Josef Stepanek, CSc., UK-MFF Flokulace je vratny jev, pfi kterem kvasinky vytvafeji shluky a urychluji tak svou sedimentaci na konci hlavniho kvaseni piva. Nami vyvinuty jednoduchy flokulacni test by mel napomoci k lepsimu poznani a diagnostice pfipadnych negativnlch zmen tohoto procesu. Tento flokulacni test umoznuje sledovat prubeh flokulace od sameho pocatku, kd)' jsou splneny vsechny podminky flokulace - pfitomnost Ca2+ v niediu i dostatek srazek mezi bunkami, az po sedimentaci zbylych volnych bunek. Navic zvoleny dmh michani dovoluje jasne rozdelit flokulacni test na cast, v ktere se postupne formuji shluky kvasinek, dale na kratky casovy usek, behem nehoz se shluky kvasinek usadi, a zaverecnou cast, kdy sedimentuji jednotlive bunky. Druha cast prace byla venovana zkoumani sedimentace a vitality deseti kmenu pivovarskych kvasnic (3, 6, 8, 9, 11, 12, 26, 32, 95 a 98). Na zaklade konfrontace mefeni velikosti bunek pod mikroskopem s casovym x'yvojem poklesu zakalu suspenze kvasnic behem sedimentace jsme sestavili kfivku, pomoci...
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Study of the performance of microbial MDR pumps by fluorescent probes: effect of potential inhibitors
Kodedová, Marie ; Gášková, Dana (advisor) ; Höfer, Milan (referee) ; Sychrová, Hana (referee)
The current increased use of antifungal agents has resulted in the development of resistance to these drugs. Search for new antifungals with different mechanisms of action overcoming the multidrug resistance is thus underway. Surface-active antifungals have the advantages of minimizing host toxicity and the emergence of drug resistance. We have developed a fluorescence method based on the use of the potentiometric fluorescent probe diS-C3(3), substrate of two major S. cerevisiae MDR pumps, Pdr5p and Snq2p. It allows us to monitor with high sensitivity and in real time changes in the activities of both pumps and also in membrane potential. We present here an efficient strategy for identifying pump inhibitors with minimal side effects on membrane integrity, and compare the potencies of different inhibitors towards MDR pumps. New efficient inhibitors of MDR pumps could potentially be used in conjunction with current antimicrobials that are MDR pump substrates. The method can be also used to determine the mechanism of action of surface-active drugs and their lowest effective concentrations.
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A study of sedimentation and flocculation of selected yeast strains by methods of complex turbidimetric analysis
Kodedová, Marie
rigorozni prace Ndzevprdce: Studium sedimentace a flokulace vybranych kmenu kvasnic metodami komplexni turbidimetricke analyzy Aulor: Mgr. Marie Kodedova Vedouciprace: Doc. Ing. Petr Sladky, CSc,, UK- MFF Komulianti: Doc. KNDr. Blanka Janderova, CSc., UK- PfF Prof. RNDr. Josef Stepanek, CSc., UK-MFF Flokulace je vratny jev, pfi kterem kvasinky vytvafeji shluky a urychluji tak svou sedimentaci na konci hlavniho kvaseni piva. Nami vyvinuty jednoduchy flokulacni test by mel napomoci k lepsimu poznani a diagnostice pfipadnych negativnlch zmen tohoto procesu. Tento flokulacni test umoznuje sledovat prubeh flokulace od sameho pocatku, kd)' jsou splneny vsechny podminky flokulace - pfitomnost Ca2+ v niediu i dostatek srazek mezi bunkami, az po sedimentaci zbylych volnych bunek. Navic zvoleny dmh michani dovoluje jasne rozdelit flokulacni test na cast, v ktere se postupne formuji shluky kvasinek, dale na kratky casovy usek, behem nehoz se shluky kvasinek usadi, a zaverecnou cast, kdy sedimentuji jednotlive bunky. Druha cast prace byla venovana zkoumani sedimentace a vitality deseti kmenu pivovarskych kvasnic (3, 6, 8, 9, 11, 12, 26, 32, 95 a 98). Na zaklade konfrontace mefeni velikosti bunek pod mikroskopem s casovym x'yvojem poklesu zakalu suspenze kvasnic behem sedimentace jsme sestavili kfivku, pomoci...
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