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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Distribution and importance of invasive pathogen Cryptostroma corticale on Acer pseudoplatanus in Prague
 Kelnarová, Ivana ; Koukol, Ondřej (advisor) ; Sumíková, Taťána (referee)
Sooty bark disease (SBD) caused by pathogen Cryptostroma corticale is one of the current risks to the population of sycamores in Europe. A few dozen individuals were fallen death due to sooty bark disease from the first record in Prague in 2005. However a higher rate of infection in a latent non-symptomatic stage can be assumed. The main goal of the thesis was to quantify the extent of infection C. corticale in Prague on the basis of early detection in tissues of symptomless trees and identify its environmental requirements and stress factors conditioning the progression of the disease. Another goal was to predict the risk for Prague's population of sycamore based on obtained data and identify other vulnerable sites in Prague. A secondary aim was to assess the spectrum of fungi inhabiting xylem of sycamore. Tissue samples collected with an increment borer were subjected to: a) isolation of cultivable fungi on malt agar and b) detection of the DNA C. corticale based on nested PCR with species- specific primers. Latent infection was detected in 28 of the cores. Of 27 positive detections with primer specific nested PCR 10 were supported by isolation of C. corticale on agar plates. Only in one case positive isolation of C. corticale on agar plate was not confirmed by nested PCR. Occurrence of C....
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Distribution and importance of invasive pathogen Cryptostroma corticale on Acer pseudoplatanus in Prague
 Kelnarová, Ivana ; Koukol, Ondřej (advisor) ; Sumíková, Taťána (referee)
Sooty bark disease (SBD) caused by pathogen Cryptostroma corticale is one of the current risks to the population of sycamores in Europe. A few dozen individuals were fallen death due to sooty bark disease from the first record in Prague in 2005. However a higher rate of infection in a latent non-symptomatic stage can be assumed. The main goal of the thesis was to quantify the extent of infection C. corticale in Prague on the basis of early detection in tissues of symptomless trees and identify its environmental requirements and stress factors conditioning the progression of the disease. Another goal was to predict the risk for Prague's population of sycamore based on obtained data and identify other vulnerable sites in Prague. A secondary aim was to assess the spectrum of fungi inhabiting xylem of sycamore. Tissue samples collected with an increment borer were subjected to: a) isolation of cultivable fungi on malt agar and b) detection of the DNA C. corticale based on nested PCR with species- specific primers. Latent infection was detected in 28 of the cores. Of 27 positive detections with primer specific nested PCR 10 were supported by isolation of C. corticale on agar plates. Only in one case positive isolation of C. corticale on agar plate was not confirmed by nested PCR. Occurrence of C....
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Phytopathogenic fungi causing necrosis of Acer pseudoplatanus bark
 Kelnarová, Ivana ; Koukol, Ondřej (advisor) ; Černý, Karel (referee)
Bark necrosis is a symptom of variously serious diseases caused by various pathogens. Sycamore is relatively susceptible to bark necrosis, especially trees weakened by stress such as climatic extremes or anthropogenic pollution. Causal agents are Ascomycetes and also Peronosporomycetes of the genus Phytophthora. Degree of pathogenicity is variable, ranging from local necrosis to cankers invading cambium resulting in death of the host. Pathogens can be classified as opportunistic (Cryptostroma corticale), weak (Prosthecium pyriforme) or obligate (Eutypella parasitica). Saprophytes decomposing dead tissue can also be isolated from necroses. To prove non-pathogenicity of these species inoculation experiments on the host are needed. Mycophagous fungi may grow in necroses as hyperparasites (Acremonium sp.). Various species of invertebrates search for necroses as a source of food and shelter. Several small mammals were observed feeding on bark necroses. Their role in spreading spores and initiating a gateway for infection is also considered.
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