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Assessment of chelation of cupric ions with flavones by use of hematoxylin method
Tamborová, Barbora ; Karlíčková, Jana (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Barbora Tamborová Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Assessment of chelation of cupric ions with flavones by use of hematoxylin method Copper is an essential trace element involved in many physiological processes. It is a part of several enzymes. Disruption of copper homeostasis results in pathological changes and diseases, excess of copper is highly toxic for human body. Flavonoids, the ubiquitous plant secondary metabolites, are highly abundant in the human diet. They represent a large group of polyphenolic compounds. They are characterized by a broad spectrum of biological activities. The anti-inflammatory, antiallergic, antimicrobial, hepatoprotective, immunomodulatory, cytotoxic and anticancer effects of flavonoids are well documented. Flavones belong to the group of flavonoids, they have chelating and antioxidative properties. They are known for their anti-inflammatory, antibacterial, antiviral and antiallergic activity. This thesis deals with chelating activity of six selected flavones, substituted on the A ring, namely 5-hydroxyflavone, baicalein, baicalin, chrysin, mosloflavone and negletein. Individual analyses were preformed using...
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Ecotoxicological evaluation of biologically active substances
Čeganová, Renáta ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Bc. Renáta Čeganová Supervisor: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: Ecotoxicological evaluation of biologically active substances For the observation of the ecotoxicological effects was selected griseofulvin that can be used as antifungal drug and local anesthetic dibucaine hydrochloride. Their analysis was performed by using a multigenerational test with a protozoan Tetrahymena thermophila, Rotoxkit F test with a rotifer Brachionus calyciflorus and algal test with Desmodesmus subspicatus. The mortality or inhibitory effects of the test substances on the growth of organisms was observed. The most sensitive of the test organisms for dibucaine hydrochloride was rotifer Brachionus calyciflorus and for griseofulvin it was alga Desmodesmus subspicatus. Keywords: ecotoxicity, biologically active substances, endpoint, biotests
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Plants as source of coumarins
Staszková, Barbora ; Karlíčková, Jana (advisor) ; Vytlačilová, Jitka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botanic and ecology Candidate: Barbora Staszková Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Plants as source of coumarins Plants are source of a wide range of substances called secondary metabolites. These substances include also coumarins, derivatives of a lactone of o-hydroxycinnamic acid. They are found in many plant species and are specific especially for the family Apiaceae, Rutaceae or Fabaceae. They can be produced by some microorganisms. They have many effects in the treatment of vascular, gastrointestinal or infectious diseases. Properties, uses and sources some of these are the subject of this diploma thesis. KEYWORDS: coumarins, secondary metabolites, effects
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Copper reducing effects of flavones
Jeřábková, Markéta ; Karlíčková, Jana (advisor) ; Filipský, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradci Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Markéta Jeřábková Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Copper reducing effects of flavones Free radicals are commonly present in our organism, however, in case of their accumulation it could cause many diseases. Copper is an important trace element which is essential for many biological processes. On the other hand, copper can catalyze processes which produce damaging radicals. Flavonoids are polyphenolic compounds with many biological effects. The most important is antioxidant activity which protects us from free radicals. Flavonids can also have a prooxidant effect. This thesis was focused on a detection of the reducing activity of flavones (mosloflavone, luteolin, apigenin, crysin, diosmin, 5-hydroxyflavone, baicalin, baicalein, negletein, apigenin-7-glucoside) at different pH. A reducing activity of surveyed flavones was taken by the methodics of spectrophotometric analysis with the use of a selective indicator of cuprous ionts formed in bathocuproinedisulfonic acid disodium salt at different pH. A reducing activity depends on the structure of flavones and pH environment. From the definition of the relation between the structure of a flavone...
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Contemporary using of medicinal Cannabis in the Czech Republic
Houdková, Tereza ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Tereza Houdková Supervisor: Prof. RNDr. Luděk Jahodář, Csc. Title of doctoral thesis: Contemporary using of medicinal Cannabis in the Czech Republic Cannabis. The plant, which is seen quite controversial. The plant, which has an excellent therapeutic effects, on the other hand, a drug that is frowned upon by many people. With the coming of legalization of therapeutic cannabis in the Czech Republi, the issue gets into the public awareness. The aim of this work is the mapping of legislation and the attitudes of institutions to the official use of cannabis as a drug substance.
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Evaluation of alkaloids using in vivo tests with Artemia salina I.
Balíková, Barbora ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Barbora Balíková Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: The evaluation of alkaloids using in vivo tests with Artemia salina I. Alzheimer's disease is the most common cause of dementia and it is one of the lifestyle diseases. There is so far available only limited symptomatic treatment and the occurrence of the lifestyle diseases (and thus Alzheimer's disease) is increasing in recent decades. Therefore it is necessary to find new compounds which will be effective in the treatment of this disease. Toxicological screening - which was our task - is the part of the research of each substance. For the evaluation of the acute toxicity appears as a suitable organism brine shrimp Artemia salina, which was used in the experiment for the purposes of this work. The experiment was conducted in a miniaturized environment. There were tested three substances belonged to the isoquinoline alkaloids: californidine, papaverine and morphine. As the most toxic was evaluated alkaloid californidine, alkaloid papaverine had less toxicity. Morphine appeared essentially as nontoxic with using the same methodology and similar concentrations as in the case of californidine...
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Antiproliferative effect of Myrica rubra extracts in cancer cell lines
Štohanslová, Barbora ; Skálová, Lenka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Barbora Štohanslová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: Antiproliferative effect of Myrica rubra extracts in cancer cell lines Myrica rubra is a woody herb planted in south-eastern Asia, especially in China. Berries, leaves and bark of Myrica rubra plant have been used in Chinese folk medicine for hundreds of years. Recent studies established significant biological effect of Myrica rubra extracts in some of the cancer cell lines. The aim of this study was to test anticancer activity of essential oil from Myrica rubra leaves (MO) to gastrointestinal cell lines (CACO-2 and HCT-8) and study mechanisms of MO effect (production of free radicals and apoptosis activation). Other goal was to compare antiproliferative effect of essential oil's fraction and α-humulene a β-caryophyllene, two most common components found in MO. Proliferation were evaluated by three different methods (NRU, MTT, x-Celligence), quantification of oxidative stress was researched using DCF. Apoptotic marker expression was examined by western blot. Results showed Myrica rubra oil inhibited the proliferation of investigated cell lines; CACO-2 seems to be more sensitive. From acquired...
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Ecotoxicological screening of diazepam drug
Šilhavá, Tereza ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
54 Abstract (EN) Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Tereza Šilhavá Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of Thesis: Ecotoxicological screening of diazepam drug Nowadays, information about the influence of medicinal products on the environment is still missing. We have studied the effect of diazepam tablet (5 mg) and the standard on the following organisms: Brachionus calyciflorus (Rotoxkit F Chronic), Thamnocephalus platyurus (Thamnotoxkit FTM ), Desmodesmus subspicatus (algal growth inhibition test). Brachionus calyciflorus was found out as the most sensitive organism also in comparison with already performed tests within the diploma thesis (Rapidtoxkit, Growth inhibition test - Tetrahymena pyriformis, Growth seeds inhibition test - Sinapis alba). Key words: ecotoxicology, diazepam, Brachionus calyciflorus, Thamnocephalus platyurus, Desmodesmus subspicatus
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Copper reducing properties of flavanones
Koblásová, Anna ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
1 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Anna Koblásová Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of the Rigorous Thesis: Copper reducing properties of flavanones Copper is an essential trace element, an integral part of many important enzymes involved in a number of vital biological processes. It is necesarry for optimal antioxidant defense of organism. Excess or deficiency of copper cause impaired cellular functions. High concentrations of copper may increase oxidative damage to lipids, proteins, and DNA and contribute to neurodegenerative disorders. Copper deficiency decreases the body's ability to deal with oxidative stress. Free copper ions may catalyze the formation of highly reactive hydroxyl radicals, for that reason they are tight binding to protein carriers. Cuproenzymes are important members of the human antioxidant system. Flavonoids are a large group of polyphenolic compounds with antioxidant and chelating properties. They inhibit lipid peroxidation, formation of reactive oxygen species and chelate redox-active metals. These abilities are influenced by the number, positions, and types of substitutions. Flavonoids have generated an interest because of their broad pharmacological...
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The content of anthocynanidines and other pigments in commercial drugs and food supplements
Karanská, Hana ; Martin, Jan (advisor) ; Karlíčková, Jana (referee)
Name of the work: The content of the anthocyanidines and other pigments in commercial drugs and food supplements Author: Hana Karanská This thesis is focused on the determination of anthocyanins and anthocyanidins in food supplements, fruits and juices. The substances were extracted with methanol using an ultrasonic bath or boiling water bath. Analysis was performed by HPLC. The results of this work show that the content of anthocyanins and anthocyanidins are in the determined samples represented in varying degrees. All the analysed samples showed the content of these substances. Based on information from the manufacturer we can say that the product Ostrovidky plus contained the declared amount of anthocyanins. Manufacturers of remaining specimens does not provide sufficient information about the composition for unambiguous determination of the result, but we can say that the products approximately satisfy the content of substances declared by the manufacturer. The amount of anthocyanins in the fruit meets the values reported in literature. Juices did not indicate the exact amount of anthocyanins.
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