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The role of ageing in the changes of the brain extracellular matrix and extracellular space properties
Kamenická, Monika ; Vargová, Lýdia (advisor) ; Růžička, Jiří (referee)
The process of aging causes the major changes in nervous tissue such as changes in the size of brain, architecture of glial cells and extracellular matrix. The size of brain is on the decrease as consequence of aging and there is a change of molecules as well as morphology at all levels. Extracellular space (ECS) is interstitium important especially in communication between cells mediated by diffusion. The limit of diffusion in extracellular space is given by size of ECS, which is discribed by volume fraction and tortuosity, that reflect amount of diffusion barriers. The changes of ECS diffusion parameters during aging were measured by real-time iontophoretic method in four parts of brain (cortex - Cx, hippocampus - Hp, inferior colliculus - IC and corpus trapezoideum - TB). Further, we studied influence of deficiency of Bral2 link protein at differences of ECS diffusion parameters and importance of Bral2 protein at aging and regulation mechanisms of cytotoxic brain edema. Our results show, that aging leads to decreasing of ECS volume v Cx and Hp, but it was not observed in IC and TB, where the intact perineuronal nets act like protecting shield against the degenerative disease induced by aging. However, small differences in composition of perineuronal nets, deficiency of Bral2 link protein, may...
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Nervous tissue regeneration following ischemic injury in adulthood - the role of glial cells
Kamenická, Monika ; Anděrová, Miroslava (advisor) ; Kletečková, Lenka (referee)
Ischemic stroke (ischemia) is one of the most common causes of death in the world. The consequences of this disease are enormous and markedly affect the lives of patients who often are unable to live a full quality of life as before. Therefore, the current research is focused on elucidating new mechanisms that could mitigate the effects of ischemia and better regeneration of nerve tissue. This theses aims to summarize the current knowledge about neurogenesis/gliogenesis in the nerve tissue under physiological conditions, after ischemic injury and subsequent regeneration. The first chapter is focused on neurogenesis in the nervous tissue of adults. The two main neurogenic regions are described, such as subventricular zone and the gyrus dentatus in hippocampus. The following is a brief description of cells that are located in neurogenic regions and their function under physiological conditions. The second chapter focuses on gliogenesis in adult nervous tissue and describes the glial cells responsible for numerous functions in CNS. Furthermore, the functions of individual types of glial cells are listed. The third chapter gives the overview about pathophysiology of ischemia. The author tries to explain what is happening in the brain tissue during and following ischemia, what types of ischemia are...
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The role of ageing in the changes of the brain extracellular matrix and extracellular space properties
Kamenická, Monika ; Vargová, Lýdia (advisor) ; Růžička, Jiří (referee)
The process of aging causes the major changes in nervous tissue such as changes in the size of brain, architecture of glial cells and extracellular matrix. The size of brain is on the decrease as consequence of aging and there is a change of molecules as well as morphology at all levels. Extracellular space (ECS) is interstitium important especially in communication between cells mediated by diffusion. The limit of diffusion in extracellular space is given by size of ECS, which is discribed by volume fraction and tortuosity, that reflect amount of diffusion barriers. The changes of ECS diffusion parameters during aging were measured by real-time iontophoretic method in four parts of brain (cortex - Cx, hippocampus - Hp, inferior colliculus - IC and corpus trapezoideum - TB). Further, we studied influence of deficiency of Bral2 link protein at differences of ECS diffusion parameters and importance of Bral2 protein at aging and regulation mechanisms of cytotoxic brain edema. Our results show, that aging leads to decreasing of ECS volume v Cx and Hp, but it was not observed in IC and TB, where the intact perineuronal nets act like protecting shield against the degenerative disease induced by aging. However, small differences in composition of perineuronal nets, deficiency of Bral2 link protein, may...
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Nervous tissue regeneration following ischemic injury in adulthood - the role of glial cells
Kamenická, Monika ; Anděrová, Miroslava (advisor) ; Kletečková, Lenka (referee)
Ischemic stroke (ischemia) is one of the most common causes of death in the world. The consequences of this disease are enormous and markedly affect the lives of patients who often are unable to live a full quality of life as before. Therefore, the current research is focused on elucidating new mechanisms that could mitigate the effects of ischemia and better regeneration of nerve tissue. This theses aims to summarize the current knowledge about neurogenesis/gliogenesis in the nerve tissue under physiological conditions, after ischemic injury and subsequent regeneration. The first chapter is focused on neurogenesis in the nervous tissue of adults. The two main neurogenic regions are described, such as subventricular zone and the gyrus dentatus in hippocampus. The following is a brief description of cells that are located in neurogenic regions and their function under physiological conditions. The second chapter focuses on gliogenesis in adult nervous tissue and describes the glial cells responsible for numerous functions in CNS. Furthermore, the functions of individual types of glial cells are listed. The third chapter gives the overview about pathophysiology of ischemia. The author tries to explain what is happening in the brain tissue during and following ischemia, what types of ischemia are...
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