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Enantioseparation of selected FMOC-derivatized amino acids on Chirobiotic T a Chiralpak QD-AX columns.
 Kašpárková, Marie ; Bosáková, Zuzana (advisor) ; Pacáková, Věra (referee)
In this work, an enantioselective HPLC method with fluorimetric detection (λEx = 254 nm, λEm = 314 nm) was developed and subsequently optimized for chiral separation of four amino acids (D/L-alanine, D/L-valine, D/L-leucine, D/L-isoleucine) in derivatized form with an emphasis on enantioseparation of D-analogues. Derivatization of amino acids was performed using 9-fluorenylmethyl chloroformate (FMOC-Cl). Retention and enantioseparation behavior of studied analytes was investigated on chiral stationary phases based on teicoplanin (Chirobiotic T) and quinidine derivative (Chiralpak QD-AX). Enantioseparation of FMOC-derivatized amino acids was performed in polar- organic separation mode on Chirobiotic T and in reverse-phase and polar-organic separation modes on Chiralpak QD-AX. Both columns were mutually compared. The Chiralpak QD-AX column in combination with polar-organic separation mode (methanol/acetonitrile/acetic acid/ammonium acetate 80/18/2/0.1 (v/v/v/w)) was shown to be the most suitable for separation of mixture of FMOC-amino acids. Under the optimized conditions quantitation of the individual FMOC-D- and FMOC-L-enantiomers, as well as a mixture of FMOC-D- and FMOC-L- enantiomers was performed. Limits of detection ranged from 15.1 to 31.3 ng/ml for FMOC-D-amino acids and from 27.7 to 48.8...
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See also: similar author names
7 KAŠPÁRKOVÁ, Martina
3 KAŠPÁRKOVÁ, Michaela
7 Kašpárková, Martina
3 Kašpárková, Michaela
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