|
|
Autorita učitelů na střední škole
Houserová, Jana
Abstract The bachelor´s thesis is focused on the perception of the authority of teachers in secondary schools. The work is divided into theoretical and practical part. The aim of the theoretical part is to define the concept of authority, the division of types of authorities, the comparison of authority at various levels of schools. It tries to describe the personality of the teacher and focuses on the relationship between students and secondary school teachers. In the theoretical part, I compared and analysed theoretical knowledge from various professional sources. The aim of the practical part is to find out how students at secondary vocational schools perceive the concept of authority in the secondary school environment, what they imagine behind it. What personalities of educators as bearers of authority they prefer and how they approach to them. I also focused on the view of students, how they look at the characteristics of teachers and the differences of opinions with respect to gender. Based on the collection of data from the questionnaire survey, evaluation, and analysis of data, I tried to propose recommendations for pedagogical practice. The research was carried out by a questionnaire, whose respondents were students at secondary vocational schools.
|
|
| |
|
|
Study of the interaction of receptor NKp46 with adhesin Epa1
Houserová, Jana ; Vaněk, Ondřej (advisor) ; Pavlíček, Jiří (referee)
One of the key components of the innate immune system are natural killer (NK) cells. The task of these cells is to induce apoptosis in target cells (e.g., cancer or virally infected cells). The target cells are identified by their interaction with surface receptors of the NK cells. On the surface of the NK cells, there are activating and inhibiting receptors. One of the activating receptors is the natural cytotoxicity receptor NKp46. Several ligands of this receptor have been identified, one of them being the epithelial adhesin Epa1 of yeast Candida glabrata. The invasive candidiasis caused by this yeast is a feared complication for patients with haematological diseases. The use of the NK cells in immunotherapy includes bispecific fusion proteins which can bind to the NK receptor with one part and to tumour antigen with the other part. This work focuses on recombinant preparation of the NKp46 protein. To facilitate a study of the effects of O-glycosylation on the binding of the ligands, a mutation of the glycosylation site NKp46 T225A was prepared. A stably transfected HEK293S GnTI- and HEK293T cells had been prepared and these proteins were then extracellularly secreted. The Epa1 protein had been produced in E. coli bacterial expression system and purified. The binding ability of the Epa1 protein...
|
|
|
Study of the interaction of receptor NKp46 with adhesin Epa1
Houserová, Jana ; Vaněk, Ondřej (advisor) ; Pavlíček, Jiří (referee)
One of the key components of the innate immune system are natural killer (NK) cells. The task of these cells is to induce apoptosis in target cells (e.g., cancer or virally infected cells). The target cells are identified by their interaction with surface receptors of the NK cells. On the surface of the NK cells, there are activating and inhibiting receptors. One of the activating receptors is the natural cytotoxicity receptor NKp46. Several ligands of this receptor have been identified, one of them being the epithelial adhesin Epa1 of yeast Candida glabrata. The invasive candidiasis caused by this yeast is a feared complication for patients with haematological diseases. The use of the NK cells in immunotherapy includes bispecific fusion proteins which can bind to the NK receptor with one part and to tumour antigen with the other part. This work focuses on recombinant preparation of the NKp46 protein. To facilitate a study of the effects of O-glycosylation on the binding of the ligands, a mutation of the glycosylation site NKp46 T225A was prepared. A stably transfected HEK293S GnTI- and HEK293T cells had been prepared and these proteins were then extracellularly secreted. The Epa1 protein had been produced in E. coli bacterial expression system and purified. The binding ability of the Epa1 protein...
|
|
|
Recombinant expression of protein NKp46 and its ligand Epa1
Houserová, Jana ; Vaněk, Ondřej (advisor) ; Kubíčková, Božena (referee)
Natural killer cells (NK cells) are cells of innate immunity that play an essential role in the immune response of an organism. In contact with infected, stressed or tumour cells, the NK cells can trigger cytotoxic mechanisms. The initiation of mechanisms depends on the presence of activating or inhibitory ligands on the surface of the cells. On the surface of the NK cells, there are activating and inhibiting receptors that upon binding their respective ligands send a signal to the NK cell. One of the activating mechanisms is the decrease of expression in MHC gp I molecules on the surface of the infected cells. This molecule is a ligand of the inhibiting receptors. One of the activating receptors of NK cells is NKp46. This receptor belongs to the natural cytotoxicity receptor (NCR) family. NKp46 has many ligands, one of them being the adhesin Epa1 of yeast Candida glabrata. This thesis aims at preparing plasmids and producing extracellular domains of the NKp46 receptor and its ligand adhesin Epa1.The interaction of NKp46 and its ligand Epa1 remains to be the subject of future research. Plasmids containing NKp46 and Epa1 genes were successfully prepared and verified by DNA sequencing. The NKp46 protein was produced in eukaryotic expression system of HEK293S GnTI- cell line. A stably transfected...
|
|
|
Performance system of employees in a financial institution
Houserová, Jana ; Kirovová, Iva (advisor) ; Uhlář, Pavel (referee)
This master thesis discusses employees appraisal in a selected organization. The theoretical part is devoted to expressions from the area of staff appraisal. Topics such as performance management, performance appraisal and work performance are clarified. Further the common process of employees appraisal is described, including its meaning and functionality. appraisal criteria are specified and selected appraisal metods are described. A specific chapter is devoted to forced distribution method. In the end of this part characteristics of high quality and effective employees appraisal are listed. In the empirical part a specific organization is presented, including description and analysis of used employees appraisal system. The goal of the research is through the analysis of the employees appraisal system and with the help of interviews with its employees determine the characteristics of a quality employees appraisal system. Based on the results of the conducted research recommendations leading to higher efectivity of employees appraisal are formed. Keywords: performance appraisal, performance management, appraisal metods, forced ranking
|
|
| |
|
| |
|
|
Si-obsahující Lavesovy fáze
Houserová, Jana
We performed electronic structure calculations to study the thermodynamic and structural properties of the C14 Laves phases in several systems with various compositions. In the frame of a thermodynamic study, we have evaluated the total energies of formation of Laves phase structure with different occupations of sublattices (Si2Si, Si2X, X2Si, X2X, where X stands for Cr, Fe, Mo and W) with respect to the Standard Element Reference (SER) states.
|
|
|
Analýza elektronové struktury Lavesových fází obsahujících chrom
Houserová, Jana ; Šob, Mojmír
Binary systems containing chromium can be found in many kinds of steels where the Laves-phase can be considered as a potential candidate for strengthening provided size and distribution of the particles are within certain limits. We performed electronic structure calculations to study the thermodynamic and structural properties of the C14 Laves phase with various compositions. In the frame of the thermodynamic study, we have evaluated the total energies of formation of Laves phase structure with different occupations of sublattices (Cr2Cr, Cr2X, X2Cr, X2X, where X stands for Fe, Mo, Si, Ta and W) with respect to the Standard Element Reference (SER) states. These energies of formation are of great importance for prediction of stability of studied configurations.
|
guest ::
RSS feed.