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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Analysis of abiotic stress induced genes in rape
 HOŠTIČKOVÁ, Irena
Breeding for abiotic stress tolerance is one of main topics in plant breeding. Oilseed rape breeding programs were for a long time focused on morphological and physiological parameters. In this thesis few experiments focused on identification of genes involved in abiotic stress reaction were performed using RT-qPCR (quantitative reverse transcription PCR). Simultaneously SPR (surface plasmon resonance) method were used as modern optical method facilitating very low native protein concentration even in presence of other substances. This method facilitates quantification of concrete proteins by binding them to specific antigen and in oilseed rape research it was not used by now. ERD10 protein was identified by SPR as protein involved in cold stress reaction (or acclimation). The results show ERD10 accumulation in standard conditions affects dynamics of its accumulation change during cold stress. In case we are searching for genotypes great in acclimation ability even during short and warm autumn SPR method should be suitable method for fast, easy and relatively cheap screening of large number of genotypes in breeding collections. Also genes LTI78, RCI2A, NRP1 and two genes for hypothetical proteins were analysed. Their relative expression during cold stress was markedly increased too. Very little is known about these genes and proteins nowadays therefor it will be interesting topic of our oncoming experiment. Relative expression of genes picked according to MALDI-TOF/TOF analysis results was also tested in microspore embryo regenerants stressed by simulated drought. Genes for lactoylglutathione lyase I, phospholipase D 1 and peroxiredoxin antioxidase were tested. In tolerant cultivar was markedly decreased gene expression of peroxiredoxin antioxidase in standard conditions and early stress. These gene will be subject for next research as potential marker for more tolerant genotypes selection.
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Influence of environmental factors on stomatal development in epidermis of Arabidopsis thaliana - signal trasduction.
 HOŠTIČKOVÁ, Irena
The stomatal function and genetically based regulation of their number are interesting and important subjects of scientific research. Stomatal density on plant leaf surface is affected also by environmental factors, e.g. atmospheric CO2 concentration and irradiance (PAR). These two factors have common affect ? leaf internal CO2 concentration (Ci) in the leaf can be changed. The question might be if the Ci is the signal for expression of genes controlling stomatal development. According to the hypothesis which this thesis is based on I proposed an increase of expression of positive regulator STOMAGEN in the case when Ci is reduced. The expression of STOMAGEN was increased in plants with Ci decreased by higher PAR in contrast with plants where the Ci was decreased by adding of abscisic acid into nutrient solution. These results show no dependence of STOMAGEN expression on inner CO2 concentration.
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Stomatal development under variable environmental conditions
 HOŠTIČKOVÁ, Irena
It is necessary for plants to adapt to variable environment conditions to be able to grow and to propagate themselves. One of the most important environmental factors is water availability. The main role in regulation of transpiration play stomata ? microscopic pores, which control gas exchange (mainly CO2, H2O, O2) between internal and external leaf environment. Number of stomata at the leaf epidermis is influenced by lots of environmental factors, e.g. atmospheric CO2 concentration, irradiance, air humidity, temperature etc. In this thesis I was aiming to study the influence of atmospheric CO2 concentration on stomatal development of Lepidium sativum L. and the influence of irradiance on stomatal development in Hordeum vulgare L.
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See also: similar author names
1 Hostičková, Iva
1 Hoštičková, Ivana
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