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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A. ; Bjerkestrand, R. ; Bjelland, B. ; Blystad, D.-R. ; Bobrova, O. ; Fránová, Jana ; Faltus, M. ; Hamborg, Z. ; Hammond Hammond, S. D. ; Koloniuk, Igor ; Zámečník, J.
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
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Virus eradication method by cryopreservation tool - a cryoknife
Bilavčík, A. ; Bobrova, O. ; Faltus, M. ; Fránová, Jana ; Hammond, S. ; Koloniuk, Igor ; Zámečník, J.
Currently, in the nursery production of plant material, emphasis is placed on the propagation of healthy material. For this reason, it is necessary to pay attention to the topic of healthy virus-free nuclear stock when preserving collections of genetic resources of vegetatively propagated plants. Some raspberry viruses are difficult to remove from the plant host by existing methods of thermotherapy or chemotherapy. That is why, using the example of black raspberry necrosis virus (BRNV), a comprehensive methodology for testing health status and cryotherapy in raspberry was developed. The resulting methodical procedure makes it possible to eradicate BRNV from raspberry using cryotherapy of in vitro cultures. The user of the methodology is the Ministry of Agriculture, which applies it as part of the 'National Program for the Conservation and Use of Genetic Resources of Plants, Animals and Microorganisms Important for Nutrition and Agriculture'.
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Olympism with respect to the Intellectual Property law
Fráňová, Jana ; Holcová, Irena (referee) ; Křesťanová, Veronika (referee)
Olympism with respect to the Intellectual Property law Intellectual property is a branch of law affecting, with various intensity, a lot of fields of human life and, together with technical development and globalisation of human life, is becoming more and more topical, which, vice versa, implicates an intensive development of the branch of law itself. Olympism is a traditional concept evoking sports ideas, profound experience of performance based on physical and mental preparedness and capacity, emotions and in a way also a life philosophy and intellectual values, but also, more and more, a significant economic factor. This thesis is based on a strong respect to both of these concepts, namely to the intellectual property and to the branch of law studying this field, analysing and developing it, and to the Olympism and its persisting undisputable sense and effect, and on the conviction about coherence of these notions both in terms of law and in terms of ordinary human life. The aims of this thesis is to set up a basic outline and to determine conceptual features of each of these both institutes especially, and, after that, to present basic forms and methods of their coherence. In this connection it was also necessary to examine the legal protection of both of these institutes, both at the national...
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Optimized biological method for the detection of sensitivity of red clover varieties to white clover mosaic virus (WClMV) and red clover mottle virus (RCMV) infection in the climatic conditions of the Czech Republic
Jakešová, H. ; Fránová, Jana
Red clover is a very important leguminous plant used in crop rotations on arable land, in temporary as well as permanent clover and grass stands. The persistence of clovers is negatively influenced by pests and diseases that attack the root and shoot parts of the plant. In the course of studies into the presence of viral diseases (2007–2011), the most frequently detected viruses were White clover mosaic virus, (WClMV, genus Potexvirus, family Alphaflexiviridae) and Red clover mottle virus (RCMV, genus Comovirus, family Secoviridae). The two viruses were observed in populations of clover throughout the Czech Republic. RCMV significantly reduced the yield and stand persistence of red clover. Given that no direct protection has yet been developed against the viruses, plant breeding directed to enhancing resistance to both WClMV and RCMV offers good prospects. Both viruses are mechanically transmissible.
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The role of credit rating agencies in evaluating risks
Fraňová, Jana ; Witzany, Jiří (advisor) ; Le, Simona (referee)
In this thesis I deal with role of rating agencies in the context of today's financial crisis. In particular, I focus on the segment evaluation of credit rating structured securities, the three largest credit rating agencies. The main part of this work include: financing credit rating agencies and regulation of credit rating agencies in the U.S. and the EU, securitization and the role of rating agencies and analysis of default studies of rating agencies. The last part is the comparison of default rates of structured finance segment by rating notches and comparison of volatility ratings of structured securities by rating notches selected the rating agencies. The time frame I set in the period from 2005 to 2012, which is evident during the financial crisis and its impact on changes in notches and default rates.
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Men's Mammography Specialities
FRÁŇOVÁ, Jana
Every male patient coming for a breast examination has already experienced some problems with the breast. In most cases they relate to the enlargement of the mammary gland ? gynaecomastia, and less often a tumour. Breast carcinoma represents only a small part of tumours among the male population with an incidence of approximately 44 new patients per year, which is less than 1% (exactly 0.13%) of malign tumours. Mammography of male patients is usually performed in the basic standard projections ? mediolateral and craniocaudal. Generally it is possible to perform other projections (but this is very rare) ? lateral and caudocranial. The mediolateral ? transversal ? projection is usually satisfactory in male patients. Due to the anatomical structure of the male breast a hands-on examination is also very important in the diagnostics of the male mammary gland. Mammographic examination is not the first-choice method. Just ultrasound examination or just mammography is satisfactory in many cases. This situation is different in female patients where mammography is the first-choice method, followed by ultrasound and biopsy in the same medical workplace. In men, mammographic or ultrasound examination can be just auxiliary to determine staging. Every workplace may perform different routines and methods in male mammographic examination. However, all these examinations should be characterised by high quality and best possible diagnostic information, professional approach of the staff, and mainly the effort to improve long-established procedures.
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Cultivated and wild growing forage crops – reservoirs of viruses and phytoplasmas
Fránová, Jana ; Petrzik, Karel ; Jakešová, H. ; Bečková, M. ; Sarkisova, Tatiana
Symptoms of mosaic, yellowing/reddening, phyllody, necrosis and dwarf have been observed in cultivated as well as wild growing forage crops. Red clover mottle virus (RCMV, Comovirus), clover yellow vein virus (Potyvirus), potato virus X, white clover mosaic virus (WClMV, Potexvirus), alfalfa latent and pea streak viruses (Carlavirus), new members of genus Cytorhabdovirus and Carlavirus were identified by sequencing. Bacilliform particles (ca 213 – 533 nm by 44 – 58 nm) of the genus Cytorhabdovirus, WClMV, alfalfa mosaic virus (Alfamovirus) and particles resembling members of the genus Badnavirus were observed on negatively stained preparations and ultrathin sections by electron microscopy and mechanically transmitted to host plants. WClMV, RCMV, bean yellow mosaic virus and new member of Potyviridae family were determined in wild growing plants. The occurrence of phytoplasmas belonging to different 16S ribosomal subgroups was confirmed (16SrI-B, 16SrI-C, 16SrX-A, 16SrXII-A).
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