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Adhesion, growth and differentiation of osteoblast-like cells on materials for bone implants
Doubková, Martina ; Bačáková, Lucie (advisor) ; Filová, Eva (referee)
This thesis focuses on testing and improving Ti-6Al-4V ELI biomaterials, which are currently one of the most used titanium alloys in biomedicine (predominantly in orthopaedics and dentistry), in cooperation with research institutions and private companies developing and producing such materials. The metallic samples were previously modified by plasma electrolytic oxidation (PEO) with use of electrolytes of a different composition to induce development of a homogeneous TiO2 layer on its surface. In vitro interactions of human osteoblast-like cell line Saos-2 with the surface of Ti-6Al-4V ELI alloy samples are investigated. Initial cell attachment, spreading, morphology, cell population density, viability, calcium deposition and expression of selected osteogenic markers, e.g. collagen type I, alkaline phosphatase and osteocalcin, were evaluated on cultured cells. The cells behavior were then correlated with physicochemical properties of the material surface, such as its topography, roughness, wettability, surface layer chemical composition etc. The results are also compared with those obtained in cells cultured on control samples of untreated alloys as well as microscopic glass coverslips and bottom of standard polystyrene cell culture wells. The aim of this thesis is to select the most promising...
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Cartilage regeneration using peroral supplementation and artificial scaffolds
Filová, Eva ; Amler, Evžen (advisor) ; Jebavý, Lukáš (referee) ; Otáhal, Stanislav (referee) ; Tománková, Kateřina (referee)
Cartilage can regenerate only in a limited extend. The aim of the study was to enhance hyaline cartilage regeneration using peroral supplementation of glycosaminoglycans (GAGs) and antioxidants, or using artificial scaffolds seeded with autologous cells. Scaffolds seeded with chondrocytes or mesenchymal stem cells (MSCs) have a potential for treatment of cartilage defects. For the complex characterization of biomechanical properties of scaffold, we developed the novel shock dynamic method. Subsequently, the composite hyaluronate-type I collagen-fibrin scaffold with the viscoelastic properties and dynamic loading response similar to the native cartilage was developed. The composite scaffold was then used for osteochondral regeneration and physeal cartilage regeneration. The effect of peroral supplementation of GAGs and antioxidants (vitamin E/selenium) on the regeneration of osteochondral defects was investigated in rabbits. After introduction of defined osteochondral defects in the knee joint, groups of ten animals were given a GAG/vitamin E/selenium mixture or a placebo (milk sugar) for 6 weeks. The amount of sulfated GAGs in the osteochondral regenerates was significantly higher in the GAG group. In both groups, the GAG amount in the cartilage of the operated knee was significantly higher than in the...
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