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Effect of selected monocyclic monoterpenes on the activity and expression of antioxidant enzymes in human liver
Fišerová, Romana ; Boušová, Iva (advisor) ; Skálová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Romana Fišerová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Title of diploma thesis: Effect of selected monocyclic monoterpenes on the activity and expression of antioxidant enzymes in human liver Monocyclic monoterpenes belong to plant secondary metabolites, which are characterized by their aroma and possess a wide range of use. They attract pollinators and are involved in plant communication as well as in the cosmetic and food industries. They are ingredients of spices, beverages, and food. In some of them, anti-cancer activity has been proven, however, we should keep in mind that some of them exert toxic properties in various organs of the organism, mainly in the liver. The aim of this thesis was to find out what effect have (+)- carvone, (-)-carvone, (+)-isomenthone, (-)-isopulegol, and piperitone on the activity and mRNA expression of antioxidant enzymes in human liver. The liver tissues obtained from patients of both sexes and 50-80 years old were used as biological material. At first, the effect of the selected monoterpenes (100 µM) on the activity of the enzymes glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferase (GST) in subcellular...
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