|
|
Advanced Fluorescence Techniques in Research on Micellar Systems and Their Interactions with Biopolymers
Holínková, Petra ; Burgert, Ladislav (referee) ; Táborský, Petr (referee) ; Pekař, Miloslav (advisor)
The dissertation thesis deals with study of advanced steady-state and time-resolved fluorescence techniques, which can be used for study of micellar systems properties. Selected fluorescence techniques were used for characterization of Septonex and CTAB cationic micellar systems and theirs interactions with hyaluronan. Fluorescent probe pyrene was used for determination of critical micelle concentration (CMC) and micellar aggregation number of these surfactants. The changes of fluorescence behaviour of fluorescein and prodan were studied in wide concentration range of Septonex. Next chapter of thesis deals with study of Förster resonance energy transfer between perylene and fluorescein in Septonex and CTAB micellar solutions and the effect of hyaluronan addition to these systems. Also steady-state and time-resolved fluorescence anisotropy studies were used for research of the effect of hyaluronan addition to micellar solutions. The last chapter of this thesis is focused on photophysical behaviour of Prodan in different solutions (water, Septonex solutions below CMC, hyaluronan solution, Septonex micellar solution and Septonex micellar solution with hyaluronan), which was discussed on the basis of time-resolved emission spectra.
|
|
|
Passive microrheology of colloidal systems based on biopolymers.
Bjalončíková, Petra ; Burgert, Ladislav (referee) ; Mravec, Filip (advisor)
Diploma thesis was aimed to deal with evaluation of microrheology method in the research of biopolymer-protein. Used biopolymer was sodium hyaluronate and proteins were trypsin and chymotrypsin. For measuring of microrheology were used particles with different radius (0,5 m and 1 m). It was found, that both substances have viscous charakter. Passive microrheology is suitable for measuring the viscoelastic properties of biopolymers.
|
|
|
Microrheology in colloid systems
Hradecká, Lucie ; Burgert, Ladislav (referee) ; Mravec, Filip (advisor)
This master thesis is focused on the evaluation of the influence of particle surface properties on the results of microrheological measurements with biopolymer solutions. Hyaluronan has been chosen as negatively charged polymer, chitosan as positively charged polymer and glycerol and its solutions of various concentrations were used as homogenous model systems. Dynamic light scattering and single particle tracking microrheology were chosen from passive microrheological techniques. Particles with various surface modifications (neutral, positive surface charge and negative surface charge) were used for the experiments. The results of microrheological techniques were then compared with classic rheology and moreover the glycerol results were compared with tabulated values.
|
|
|
Technology of monofilamentous fibers based on oxidized hyaluronic acid
Běťák, Jiří ; Mráček, Aleš (referee) ; Burgert, Ladislav (referee) ; Klučáková, Martina (advisor)
Předkládaná dizertační práce se zabývá vývojem technologie výroby nového typu biodegradabilních vláken na bázi oxidované kyseliny hyaluronové. V rámci práce je postupně představován vývoj jednotlivých jednotkových operací výroby, jejichž správné porozumění a schopnost jejich řízení jsou klíčové pro žádaný chod celé vícestupňové technologie. V rámci práce je představen nezbytný vývoj technologického zařízení, průběžně konstruovaného pro účely laboratorního testování a následně až po samotnou linku pro finální výrobu vláken, která byla realizována v roce 2015. V rámci dizertační práce jsou dále navrhovány možnosti dodatečné chemické úpravy vláken s ohledem na zvyšování jejich stability ve vlhkém prostředí. S ohledem na cílené aplikace vláken pro vnitřní chirurgické implantace, jsou v práci vlákna též hodnocena z hlediska jejich materiálové biokomaptibility (toxicity).
|
|
|
Hyaluronan-Micelle Aggregates and their Potential for Nanomedicine Applications
Pilgrová, Tereza ; Burgert, Ladislav (referee) ; Reháková, Milena (referee) ; Pekař, Miloslav (advisor)
This thesis deals with the study of preparation and characterization of hyaluronan-micelle aggregates. The theoretical part deals with drug delivery systems, characterization of used materials and methods especially fluorescence spectroscopy, dynamic light scattering and turbidimetry. Methods of determination of measured data are summarized in the experimental section. The result section is divided into two subsections dedicated to different preparation methods of hyaluronan-surfactant complexes. Induced aggregates of hyaluronan with Septonex are characterized in terms of their origin and stability, and the results are compared with previously studied surfactants CTAB. In the second part are discussed so-called decorated micelles, their formation, properties and stability.
|
|
|
Advanced Fluorescence Techniques in Research on Micellar Systems and Their Interactions with Biopolymers
Holínková, Petra ; Burgert, Ladislav (referee) ; Táborský, Petr (referee) ; Pekař, Miloslav (advisor)
The dissertation thesis deals with study of advanced steady-state and time-resolved fluorescence techniques, which can be used for study of micellar systems properties. Selected fluorescence techniques were used for characterization of Septonex and CTAB cationic micellar systems and theirs interactions with hyaluronan. Fluorescent probe pyrene was used for determination of critical micelle concentration (CMC) and micellar aggregation number of these surfactants. The changes of fluorescence behaviour of fluorescein and prodan were studied in wide concentration range of Septonex. Next chapter of thesis deals with study of Förster resonance energy transfer between perylene and fluorescein in Septonex and CTAB micellar solutions and the effect of hyaluronan addition to these systems. Also steady-state and time-resolved fluorescence anisotropy studies were used for research of the effect of hyaluronan addition to micellar solutions. The last chapter of this thesis is focused on photophysical behaviour of Prodan in different solutions (water, Septonex solutions below CMC, hyaluronan solution, Septonex micellar solution and Septonex micellar solution with hyaluronan), which was discussed on the basis of time-resolved emission spectra.
|
|
|
Hyaluronan-Micelle Aggregates and their Potential for Nanomedicine Applications
Pilgrová, Tereza ; Burgert, Ladislav (referee) ; Reháková, Milena (referee) ; Pekař, Miloslav (advisor)
This thesis deals with the study of preparation and characterization of hyaluronan-micelle aggregates. The theoretical part deals with drug delivery systems, characterization of used materials and methods especially fluorescence spectroscopy, dynamic light scattering and turbidimetry. Methods of determination of measured data are summarized in the experimental section. The result section is divided into two subsections dedicated to different preparation methods of hyaluronan-surfactant complexes. Induced aggregates of hyaluronan with Septonex are characterized in terms of their origin and stability, and the results are compared with previously studied surfactants CTAB. In the second part are discussed so-called decorated micelles, their formation, properties and stability.
|
|
|
Technology of monofilamentous fibers based on oxidized hyaluronic acid
Běťák, Jiří ; Mráček, Aleš (referee) ; Burgert, Ladislav (referee) ; Klučáková, Martina (advisor)
Předkládaná dizertační práce se zabývá vývojem technologie výroby nového typu biodegradabilních vláken na bázi oxidované kyseliny hyaluronové. V rámci práce je postupně představován vývoj jednotlivých jednotkových operací výroby, jejichž správné porozumění a schopnost jejich řízení jsou klíčové pro žádaný chod celé vícestupňové technologie. V rámci práce je představen nezbytný vývoj technologického zařízení, průběžně konstruovaného pro účely laboratorního testování a následně až po samotnou linku pro finální výrobu vláken, která byla realizována v roce 2015. V rámci dizertační práce jsou dále navrhovány možnosti dodatečné chemické úpravy vláken s ohledem na zvyšování jejich stability ve vlhkém prostředí. S ohledem na cílené aplikace vláken pro vnitřní chirurgické implantace, jsou v práci vlákna též hodnocena z hlediska jejich materiálové biokomaptibility (toxicity).
|
|
|
Microrheology in colloid systems
Hradecká, Lucie ; Burgert, Ladislav (referee) ; Mravec, Filip (advisor)
This master thesis is focused on the evaluation of the influence of particle surface properties on the results of microrheological measurements with biopolymer solutions. Hyaluronan has been chosen as negatively charged polymer, chitosan as positively charged polymer and glycerol and its solutions of various concentrations were used as homogenous model systems. Dynamic light scattering and single particle tracking microrheology were chosen from passive microrheological techniques. Particles with various surface modifications (neutral, positive surface charge and negative surface charge) were used for the experiments. The results of microrheological techniques were then compared with classic rheology and moreover the glycerol results were compared with tabulated values.
|
|
|
Passive microrheology of colloidal systems based on biopolymers.
Bjalončíková, Petra ; Burgert, Ladislav (referee) ; Mravec, Filip (advisor)
Diploma thesis was aimed to deal with evaluation of microrheology method in the research of biopolymer-protein. Used biopolymer was sodium hyaluronate and proteins were trypsin and chymotrypsin. For measuring of microrheology were used particles with different radius (0,5 m and 1 m). It was found, that both substances have viscous charakter. Passive microrheology is suitable for measuring the viscoelastic properties of biopolymers.
|
guest ::
