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Assembly, successional development and functioning of microbial communities in deadwood
Bernardová, Natálie ; Brabcová, Vendula (advisor) ; Kopecký, Jan (referee)
Dead wood is one of the most important reservoirs associated with forest ecosystems. In natural forests, its volume is counted in hundreds of m3 ha-1 , whereas it reaches only tens of m3 ha-1 in productive commercial forests. In contrast to soil and plant litter, deadwood is unevenly distributed on the forest floor. The specific physicochemical properties such as high content of recalcitrant polymers, low nitrogen level and impermeability negatively affect the rate of decomposition especially in the initial stages of wood deconstruction. The deadwood decomposition is very slow in comparisons with other substrates, it accumulates and thus it represents the important reservoir of nutrients. This thesis is focused on the structure, development and function of microbial (fungal) community in decomposing deadwood in unmanaged forest. Functional screening of fungi isolated from fruit bodies collected from coarse deadwood was set aside. Physico-chemical properties of deadwood including pH, carbon and nitrogen content and microbial biomass were estimated for four wood decomposition stages and three different tree species. New generation sequencing (Illumina MiSeq platform) was applied for fungal community structure analysis based on ITS2 fragment. Fungal functional screening was based on physico-chemical...
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