|
| |
|
|
Changes in the chromatin structure during granulocytic differentiation
Bártová, Eva ; Jirsová, Pavla ; Skalníková, M. ; Kozubek, Stanislav ; Kozubek, Michal ; Lukášová, Emilie ; Cafourková, Alena ; Koutná, I. ; Paseková, R. ; Matula, Pa. ; Matula, Pe.
|
|
|
Position of centromeres in the interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation
Bártová, Eva ; Kozubek, Stanislav ; Durm, S. ; Hausmann, M. ; Kozubek, Michal ; Lukášová, Emilie ; Skalníková, M. ; Jirsová, Pavla ; Cafourková, Alena ; Buchníčková, Katarína
|
|
| |
|
| |
|
| |
|
|
Rozdílná jaderná lokalizace aberantních a normálních homologů chromosomu 8 v intaktních nebo NaBt diferencovaných buňkách HT-29
Harničarová, Andrea ; Bártová, Eva ; Kozubek, Stanislav
An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes.
|
|
|
Cytologická studie modelů DNA metylace a metylace histonů u lidských buněčných linií
Skalníková, M. ; Bártová, Eva ; Kozubek, Stanislav ; Kozubek, Michal
Epigenetic processes are defined as heritable changes in genome function that occur without a change in DNA sequence. Gene expression, chromosome segregation, DNA replication, repair, and recombination all act, not on DNA alone, but on the chromatin template. DNA methylation, along with histone lysine methylation, establishes the framework for long-term epigenetic maintenance. The discovery that enzymes can (re)organise chromatin into accessible and inaccessible configurations revealed epigenetic mechanisms that considerably extend the information potential of the genetic code. In mammals, heterochromatin is characterised by DNA methylation at CpG dinucleotides and methylation at lysine 9 of histone 3 (H3-K9), whereas euchromatin is associated with methylation at lysine 4 of histone 3 (H3-K4).
|
|
|
Klastrování genů s podobným regulačním mechanismem během monocytární a garanulocytární diferenciace buněk HL-60
Koutná, I. ; Faltýsková, E. ; Krontorád, P. ; Svoboda, Z. ; Pavlík, T. ; Bártová, Eva ; Kozubek, Michal ; Kozubek, Stanislav
In the present study, a high density-microarrays of human cDNA containing 19,000 genetic elements were used to search for differences in gene expression of human promyelic leukaemia cell line HL-60 during monocyte (MD) and granulocyte differentiation (GD). For differentiation to monocytes (granulocytes), the TPA, 12-O-tetradecanoylphorbol-D-acetate (DMSO) were used, respectively. A total number of 4625 (4760) genes were found to be regulated during MD (GD). The genes have been divided into groups according to their kinetics of regulation (R-groups). The genes of the same group are not distributed randomly throughout the genome but form clusters on chromosomes (R-clusters). Thus, functionally interconnected genes are frequently localized near to each other on the DNA molecule.
|
|
| |
guest ::
RSS feed.