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Actual health status of red deer (Cervus elaphus) population in KRNAP region
Bártl, Josef ; Lamka, Jiří (advisor) ; Ducháček, Lubomír (referee)
This study took place in 2006 and 2007 and includes the results of the 2005, is aimed at verifying the health status of wild stocks of Red Deer (Cervus elaphus) in KRNAP in the Czech Republic. The incidence of parasitic infections was investigated by coprological methods (qualitative ovoscopic method and quantitative larvoscopic method) on the Pharmaceutical Faculty of Charles University in Hradec Kralove, Department of Pharmacology and Toxicology. In all yards were from parasitic nematodes most evidenced by the frequency Elaphostrongylus cervi, with less frequency in other nematodes Strongylida family and genus Capillaria. Mycobacterial infection caused by Mycobacterium avium subsp. paratuberculosis and Mycobacterium bovis were investigated by the culture method of mycobacteriosis and serological methods immunodifuse test (IDT) and complement fixation (RVK) on Research Institute of Veterinary Medicine, Center Laboratory, accredited testing laboratory in Brno (registration number: 1354). In this study was not discovered any positive samples. The study shows that mycobacterial infection not occur in wild population of Red Deer, and the parasitic infections in gastrointestinal tract are very low.
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HPLC and LC-MS/MS methods for diagnosis of inherited metabolic diseases
Bártl, Josef
This dissertation thesis is focused on development and optimization of high- performance liquid chromatography (HPLC) and tandem mass spectrometry (LC-MS/MS) methods, and its utility for diagnosis of inherited metabolic diseases. The first thematic part describes a comprehensive laboratory approach to diagnostics of patients with hereditary xanthinuria by determination of specific markers and enzyme activity. For this purpose HPLC method with diode array detection for measurement of hypoxanthine, xanthine, allopurinol and oxypurinol in urine and plasma and HPLC method with fluorimetric detection for analysis of pterin and isoxanthopterin in plasma were employed. These methods were successfully applied in clinical practice to ascertain two patients with hereditary xanthinuria type I. The second thematic part aims at developing and clinical application of new LC-MS/MS method for simultaneous determination of total homocysteine (tHcy), methionine (Met) and cystathionine (Cysta) in dried blood spots (DBS) and plasma. The results demonstrated the clinical utility of this method for detection of patients with homocystinuria and possibility to distinguish between defects in the remethylation and transsulfuration pathways of homocysteine metabolism. Due to ease of DBS collection and sample transportation...
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HPLC and LC-MS/MS methods for diagnosis of inherited metabolic diseases
Bártl, Josef
This dissertation thesis is focused on development and optimization of high- performance liquid chromatography (HPLC) and tandem mass spectrometry (LC-MS/MS) methods, and its utility for diagnosis of inherited metabolic diseases. The first thematic part describes a comprehensive laboratory approach to diagnostics of patients with hereditary xanthinuria by determination of specific markers and enzyme activity. For this purpose HPLC method with diode array detection for measurement of hypoxanthine, xanthine, allopurinol and oxypurinol in urine and plasma and HPLC method with fluorimetric detection for analysis of pterin and isoxanthopterin in plasma were employed. These methods were successfully applied in clinical practice to ascertain two patients with hereditary xanthinuria type I. The second thematic part aims at developing and clinical application of new LC-MS/MS method for simultaneous determination of total homocysteine (tHcy), methionine (Met) and cystathionine (Cysta) in dried blood spots (DBS) and plasma. The results demonstrated the clinical utility of this method for detection of patients with homocystinuria and possibility to distinguish between defects in the remethylation and transsulfuration pathways of homocysteine metabolism. Due to ease of DBS collection and sample transportation...
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HPLC and LC-MS/MS methods for diagnosis of inherited metabolic diseases
Bártl, Josef ; Jelínek, Ivan (advisor) ; Čabala, Radomír (referee) ; Cvačka, Josef (referee)
This dissertation thesis is focused on development and optimization of high- performance liquid chromatography (HPLC) and tandem mass spectrometry (LC-MS/MS) methods, and its utility for diagnosis of inherited metabolic diseases. The first thematic part describes a comprehensive laboratory approach to diagnostics of patients with hereditary xanthinuria by determination of specific markers and enzyme activity. For this purpose HPLC method with diode array detection for measurement of hypoxanthine, xanthine, allopurinol and oxypurinol in urine and plasma and HPLC method with fluorimetric detection for analysis of pterin and isoxanthopterin in plasma were employed. These methods were successfully applied in clinical practice to ascertain two patients with hereditary xanthinuria type I. The second thematic part aims at developing and clinical application of new LC-MS/MS method for simultaneous determination of total homocysteine (tHcy), methionine (Met) and cystathionine (Cysta) in dried blood spots (DBS) and plasma. The results demonstrated the clinical utility of this method for detection of patients with homocystinuria and possibility to distinguish between defects in the remethylation and transsulfuration pathways of homocysteine metabolism. Due to ease of DBS collection and sample transportation...
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Actual health status of red deer (Cervus elaphus) population in KRNAP region
Bártl, Josef ; Lamka, Jiří (advisor) ; Ducháček, Lubomír (referee)
This study took place in 2006 and 2007 and includes the results of the 2005, is aimed at verifying the health status of wild stocks of Red Deer (Cervus elaphus) in KRNAP in the Czech Republic. The incidence of parasitic infections was investigated by coprological methods (qualitative ovoscopic method and quantitative larvoscopic method) on the Pharmaceutical Faculty of Charles University in Hradec Kralove, Department of Pharmacology and Toxicology. In all yards were from parasitic nematodes most evidenced by the frequency Elaphostrongylus cervi, with less frequency in other nematodes Strongylida family and genus Capillaria. Mycobacterial infection caused by Mycobacterium avium subsp. paratuberculosis and Mycobacterium bovis were investigated by the culture method of mycobacteriosis and serological methods immunodifuse test (IDT) and complement fixation (RVK) on Research Institute of Veterinary Medicine, Center Laboratory, accredited testing laboratory in Brno (registration number: 1354). In this study was not discovered any positive samples. The study shows that mycobacterial infection not occur in wild population of Red Deer, and the parasitic infections in gastrointestinal tract are very low.
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