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National Repository of Grey Literature

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	Vliv komensální mikroflóry na indukci slizniční tolerance k alergenu pylu břízy u BALB/c myší Kozáková, Hana ; Repa, A. ; Štěpánková, Renata ; Hrnčíř, Tomáš ; Schwarzer, Martin ; Hudcovic, Tomáš ; Tlaskalová, Helena ; Wiedermann, U. Compared with germ-free mice model, the microflora has no effect on the development of allergic responses or the ability to induce tolerance using a clinical relevant allergen Detailed record
	Vliv rekombinantní bakterie Lactobacillus plantarum na vývoj pylové alergieL Schwarzer, Martin ; Repa, A. ; Wiedermann, U. ; Hrnčíř, Tomáš ; Daniel, C. ; Pot, B. ; Štěpánková, Renata ; Hudcovic, Tomáš ; Tlaskalová, Helena ; Součková, Martina ; Kozáková, Hana Germfree mice were monocolonized with recombinant Lactobacillus plantarum producing Bet v 1. Subsequently mice were sensitized by intraperitoneal injections to Bet v 1. We found out that monocolonization with recombinant L. plantarum reduced the level of specific Bet v 1 specific IgE antibody and stimulated production of INF-gamma in spleen cells supernatants. We conclude that monocolonization by recombinant L. plantarum shifts the immune response towards Th-1 direction and that it is a promising vaccine candidate against type I allergy Detailed record
	Influence of germ-free conditions on the development of sensitization and tolerance to the main compound of brich pollen Bet v 1 in mice Kozáková, Hana ; Repa, A. ; Štěpánková, Renata ; Hrnčíř, Tomáš ; Hudcovic, Tomáš ; Tlaskalová, Helena ; Pollak, A. ; Wiedermann, U. Birch pollen is one of the most frequent airborne allergen of Central and North Europe. The effect of intestinal microflora on sensitization and mucosal tolerance to main component of birch pollen Bet v 1 did not be study yet. Germ-free and conventional mice were administrated intragastrically or intranasally with Bet v 1 following by subcutaneous injections of Bet v 1. Tolerance was established independently on microflora present resulting decreased level of IgE and changed cytokine production Detailed record
	Vnímavost k navození nazální a orální tolerance je nezávislá na přítomnosti mikroflóry Repa, A. ; Kozáková, Hana ; Hudcovic, Tomáš ; Štěpánková, Renata ; Hrnčíř, Tomáš ; Tlaskalová, Helena ; Pollak, A. ; Wiedermann, U. The development of mucosal tolerance to major allergic component of birch pollen in relationship with presence of intestinal microflora was the aim of presented study. Germ-free (GF) or conventionally reared (CV) BALB/c mice were intragastrically or intranasally pre-treated with Bet v 1 prior to sensitization performed by subcutaneous injections of Bet v 1. Oral as well as intranasal tolerance induction led to a significant reduction of allergen-specific antibody levels and IgE basophil degranulation, as well as cytokine production in vitro in both GF and CV mice. The importance of the microflora for mucosal tolerance induction in GF mice remains therefore a matter of debate Detailed record

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