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National Repository of Grey Literature

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	Quantum dot luminescent probe for Caspase 3/7 imaging inside cells Procházková, Markéta ; Klepárník, Karel Two step synthesis of luminescent probe based on ligand-exchange in the first step and the specific reaction of amino group in the second step was optimized. The luminescence properties of the final product were checked by time course of\nactive recombinant caspase protein reaction under the model conditions in fluorimeter. The novel luminescent probe enables long-time imaging of active caspases in living cells or tissues. Detailed record
	Bioluminescent probe for caspase imaging inside cells based on Foerster resonance energy transfer between quantum dot and quencher Procházková, Markéta ; Klepárník, Karel In this work testing of novel quantum dot luminescent probe is presented. The quantum dot FRET-based luminescent probe enables long-time luminescent imaging of active caspases in living cells under microscope. Detailed record
	Bioluminescence determination of caspase-3/7 in single osteosarcoma cells Killinger, Michael ; Matalová, Eva ; Klepárník, Karel The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for appropriate interpretation of diagnostic and therapeutic results. Here, we determined the level of active caspase-3/7 in physiological and pathological (cancer) conditions by our miniaturized instrumentation. Detailed record

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