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Structure and evolution of plant sex chromosomes: The use of BAC library
Kejnovský, Eduard ; Lengerová, Martina ; Hobza, Roman ; Široký, Jiří ; Vyskot, Boris
Sex chromosomes of plant species Silene latifolia are the ideal model for evolutionary biology studies because they represent early stages of X and Y chromosomes history. We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) clones in studies of chromosome structure to demonstrate that a majority of genes are located in subtelomeric regions of all chromosomes. Our results also indicate the absence of accumulation of main classes of repetitive sequences - retroelements and centromeric tandem repeats - in non-recombining parts of the sex chromosomes.
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Genomic imprinting
Vyskot, Boris
Genomic imprinting represents an epigenetic marking of genes that results in their monoallelic expression, namely in mammals and flowering plants. Here, I also summarize our recent experiments describing the role of DNA methylation and histone H4 acetylation in plant reproductive development.
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Genetic and epigenetic acpects of sex determination
Janoušek, Bohuslav ; Hodurková, Jaromíra ; Žlůvová, Jitka ; Lengerová, Martina ; Hobza, Roman ; Nix, Tomáš ; Vyskot, Boris
The article concentrates on the latest knowledge concerning the role of epigenetic mechanisms in sex determination in model dioecious species Silene latifolia. Results suggest that the locus suppressing development of female organs in male plants is hypersensitive to the action of agents influencing level of DNA methylation (5-azacytidine) and histone acetylation (trichostatin A). According to the results obtained via inter-specific hybridisation and the ability of the smut Ustilago violacea to activate development of male organs in female plants, it can be deduced that there is a locus suppressing male organ development in standard female plants. Function of this locus is not influenced by the drugs mentioned above.
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Evolution of electrochemical methods: from virtual capabilities to virtual instruments
Dražan, Viktor
Progress in electrochemical methods is tightly bound with the development of electronics and instrumentation. Modern development environments allow one to create software for measurement control, data processing and modelling without deep knowledge of computer programming. Utilisation of one of those environments, the LabView, for modelling the results of adsorption kinetics measurement on mercury drop electrode will be demonstrated in the presentation.
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